Home / RANK/TNFRSF11A / Recombinant Mouse RANK/TNFRSF11A Fc Chimera Protein, CF

Recombinant Mouse RANK/TNFRSF11A Fc Chimera Protein, CF

Catalog #: 692-RK
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
692-RK-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse RANK/TNFRSF11A Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit TRANCE-induced osteoclast differentiation of mouse splenocytes. The ED50 for this effect is 0.05‑0.15 µg/mL in the presence of 10 ng/mL of rmTRANCE.
Source
Mouse myeloma cell line, NS0-derived mouse RANK/TNFRSF11A protein
MetMouse RANK
(Gln30 - Pro213)
Accession # O35305.1		 IEGRDID Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus
Accession #
O35305.1
N-terminal Sequence
Analysis
Met
Structure / Form
disulfide-linked homodimer
Predicted Molecular Mass
48 kDa (monomer)
SDS-PAGE
60 kDa, reducing conditions

Product Datasheets

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692-RK

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

692-RK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR] and TNFRSF11A) is a member of the tumor necrosis factor receptor family. The full length mouse RANK cDNA encodes a type I transmembrane protein of 625 amino acids (aa) with a predicted 184 aa extracellular domain and a 391 aa cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% aa identity in their extracellular domains. RANK is widely expressed with the highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References
  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
Long Name
Receptor Activator of NF-kappa-B
Entrez Gene IDs
8792 (Human); 21934 (Mouse)
Alternate Names
CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Citations for Recombinant Mouse RANK/TNFRSF11A Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Development of bone seeker-functionalised microspheres as a targeted local antibiotic delivery system for bone infections
    Authors: SG Rotman, K Thompson, DW Grijpma, RG Richards, TF Moriarty, D Eglin, O Guillaume
    J Orthop Translat, 2019-08-30;21(0):136-145.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice.
    Authors: Shimamura M, Nakagami H, Osako M, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R
    Proc Natl Acad Sci U S A, 2014-05-20;111(22):8191-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Epidermal growth factor receptor (EGFR) signaling is a key mediator of hormone-induced leukocyte infiltration in the pubertal female mammary gland.
    Authors: Aupperlee M, Zhao Y, Tan Y, Leipprandt J, Bennett J, Haslam S, Schwartz R
    Endocrinology, 2014-04-02;155(6):2301-13.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts.
    Authors: Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N
    J. Immunol., 2007-01-01;178(1):192-200.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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