Recombinant Mouse Semaphorin 3D Fc Chimera Protein Summary
Product Specifications
| Mouse Semaphorin 3D (Ser38-Glu738) Accession # Q8BH34 | IEGRMDP | Mouse IgG2a (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9386-S3
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 400 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
9386-S3/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. |
| Reconstitution | Reconstitute at 400 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Mouse Semaphorin-3D (Catalog # 9386-S3) binds HUVEC human umbilical vein endothelial cells in a dose-dependent manner. The ED50 for this effect is 1.5 -9 µg/mL.
Reconstitution Calculator
Background: Semaphorin 3D
Semaphorin 3D (Sema3D) is a member of the semaphorin family that plays a role in neuronal and vascular development (1, 2). Class 3 semaphorins are secreted molecules, and contain a common domain structure. The mouse Sema3D domains consist of a 488 aa Sema domain, a 53 aa plexin-semaphorin-integrin (PSI) domain, an 89 aa Ig-like C2-type domain, and a 35 aa basic domain which is proteolytically processed (3, 4). Within these domains, mouse Sema3D shares 94% and 98% aa sequence identity with human and rat Sema3D, respectively. Semaphorin 3D is a secreted guidance molecule involved in angiogenesis and neuronal development (5). It induces the collapse and paralysis of neuronal growth cones and promotes fasciculation of axons (6, 7). Sema3D also inhibits endothelial cell motility and tubulogenesis through cytoskeletal reorganization (2). Binding to Neuropilin-I is required for Sema3D signaling (8). ErbB2 was shown to be a potential Sema3D co-receptor, which is phosphorylated in response to Sema3D in HUVEC cells (8). Semaphorin 3D inhibition of vascular development facilitates inhibition of glioblastoma growth in the cortex of the brain (1).
- Sabag, A. et al. (2012) PLoS One. 7:e42912.
- Aghajanian, H. et al. (2014) J. Biol. Chem. 289:17971.
- Takahashi, K. et al. (2009) Biochimica et Biophysica Acta. 1790:395.
- Gu, C. and E. Giraudo. (2013) Exp. Cell Res. 319:1306.
- Binch, A. et al. (2015) Oncotarget. 6:18338.
- Kuhn, T.B. et al. (2000) J. Neurobiology. 44:126.
- Wolman, M.A. et al. (2007) J. Neurobiology. 27: 9653-63.
- Aghajanian, H. et al. (2015) Nature Commun. 7:12038.
Citations for Recombinant Mouse Semaphorin 3D Fc Chimera Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Spatially organized cellular communities form the developing human heart
Authors: Farah, EN;Hu, RK;Kern, C;Zhang, Q;Lu, TY;Ma, Q;Tran, S;Zhang, B;Carlin, D;Monell, A;Blair, AP;Wang, Z;Eschbach, J;Li, B;Destici, E;Ren, B;Evans, SM;Chen, S;Zhu, Q;Chi, NC;
Nature
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B
Authors: V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness
J. Neurosci., 2019-06-10;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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