Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF
Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF Summary
Product Specifications
| Mouse Semaphorin 6B (Leu27-Ser605) Accession # O54951 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2264-S6
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Semaphorin 6B
Semaphorin 6B is a member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, and dendritic spine formation (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain the characteristic extracellular beta propeller-structured N-terminal semaphorin (sema) domain and also an extracellular plexin-semaphorin-integrin (PSI) domain (2, 3). Additionally, the cytoplasmic domain of Semaphorin 6B contains a proline-rich sequence that has been shown to interact with the SH3 domain of the Src signaling protein (4). Mouse Semaphorin 6B has a molecular weight of approximately 120 kDa and is thought to form disulfide-linked homodimers (4). It shares 95% and 99% amino acid (aa) sequence identity with the human and rat orthologs, respectively. In mice, Semaphorin 6B is highly expressed in developing muscle and in subregions of the nervous system, as well as at low levels in most adult tissues (4). It has been shown to mediate repulsion of mouse sympathetic ganglion axons and hippocampal mossy fibers via binding to the transmembrane protein Plexin A4 (5, 6). Semaphorin 6B has also been shown to be highly expressed in human gastric, breast, and glioblastoma cancer cells and is thought to be involved in tumor differentiation and metastasis (7-9).
- Pasterkamp, R.J. and R.J. Giger (2009) Curr. Opin. Neurobiol. 19:263.
- Neufeld, G. et al. (2005) Front. Biosci. 10:751.
- Roney, K. et al. (2013) Protein Cell 4:17.
- Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
- Suto, F. et al. (2005) J. Neurosci. 25:3628.
- Tawarayama, H. et al. (2010) J. Neurosci. 30:7049.
- Correa, R.G. et al. (2001) Genomics 73:343.
- Murad, H. et al. (2006) Int. J. Oncol. 28:977.
- Ge, C. et al. (2013) J. Int. Med. Res. 41:284.
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