Recombinant Mouse SEZ6/BSRP-C Protein, CF Summary
Product Specifications
Gly19-Asn902, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4989-BC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: SEZ6/BSRP-C
Seizure Related Gene 6 (SEZ6), also known as Brain-specific Receptor-like Protein C (BSRP-C), is a member of the SEZ6 family of proteins with a predicted molecular weight of 150 kDa (1, 2). It is a type I transmembrane glycoprotein composed of an extracellular domain that contains three CUB domains and five short consensus repeats (SCRs) (1-3). Alternative splicing generates two additional isoforms for SEZ6. One isoform is a transmembrane protein that shows a deletion of amino acids (aa) 895-907 plus a 10 aa substitution for aa 982-991. The second splice variant is a soluble protein that shows an 18 aa substitution for aa 588-991 (1, 2). Mouse SEZ6 shares 91% and 97% aa sequence identity with the human and rat orthologs, respectively. SEZ6 is expressed in neurons in the developing and adult mouse brain and retina (1-6). It has been suggested that BSRP-C is important for proper development of dendritic arbors and excitatory synapses. SEZ6 has been shown to inhibit neurite branching of cortical neurons with little effect on neurite extension (7). Additionally, SEZ6 has been shown to interact with Motopsin/PRSS12, a serine protease believed to be essential for the reorganization of neuronal structures in the developing and adult brain (8). Mutations in Sez6 have been associated with febrile seizures (9, 10).
- Shimizu-Nishikawa, K. et al. (1995) Biochem. Biophys. Res. Commun. 216:382.
- Miyazaki, T. et al. (2006) FEBS Lett. 580:4057.
- Shimizu-Nishikawa, K. et al. (1995) Brain Res. Mol. Brain Res. 28:201.
- Kim, M.H. et al. (2002) Mech. Dev. 118:171.
- Gunnersen, J.M. et al. (2009) PLoS One 4:e6546.
- Osaki, G. et al. (2011) Brain Res. 1386:58.
- Gunnersen, J.M. et al. (2007) Neuron 56:621.
- Mitsui, S. et al. (2013) Biochem. Biophys. Res. Commun. 436:638.
- Yu, Z.L. et al. (2007) J. Neurosci. Res. 85:166.
- Mulley, J.C. et al. (2011) Neurol. Res. Int. 2011:917565.
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