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Recombinant Mouse sFRP-1 Protein

Catalog #: 9019-SF
11 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
9019-SF-025/CF

With Carrier

Catalog # Availability Size / Price Qty
9019-SF-025
Recombinant Mouse sFRP-1 Protein Bioactivity.
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Product Details
Citations (11)
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Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse sFRP-1 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.600-7.20 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse sFRP-1 protein
Ser32-Lys314
Accession #
Q8C4U3
N-terminal Sequence
Analysis
Ser32
Predicted Molecular Mass
33 kDa
SDS-PAGE
37-43 kDa, reducing conditions

Product Datasheets

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9019-SF (with carrier)

Product Datasheet
COA
SDS

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9019-SF/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9019-SF

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9019-SF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Mouse sFRP-1 Protein (Catalog # 9019-SF) inhibits Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.600-7.20 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-1

Secreted Frizzled Related Protein-1 (sFRP-1), also known as SARP-2, FRP, and FrzA, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature mouse sFRP-1 shares 97% amino acid sequence identity with human and rat sFRP-1. It binds to Wnt-1, -2, -3a, and -4 and prevents their activation of canonical beta-catenin signaling (4-7). sFRP-1 is up-regulated in the ischemic heart where it limits inflammation and the severity of tissue damage following myocardial infarction (8-10). During angiogenesis it induces endothelial cell migration, tube formation, and vessel formation (11). sFRP-1 is expressed by bone marrow stromal cells and osteoclasts (12). It binds to TRANCE/RANK Ligand, inhibits osteoclast and osteoblast formation and survival, and inhibits the formation of trabecular bone (13, 14). sFRP-1 additionally restricts kidney tubule formation (7), the inflammatory activation of leukocytes (10), early B lymphoid progression (12), and vitreous fluid outflow in the eye (15).

References
  1. Cruciat, C.M. and C. Niehrs (2013) Cold Spring Harb. Perspect. Biol. 5:e015081.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
  4. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
  5. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  6. Wawrzak, D. et al. (2007) Biochem. Biophys. Res. Commun. 357:1119.
  7. Yoshino, K. et al. (2001) Mech. Dev. 102:45.
  8. Barandon, L. et al. (2002) Circulation 108:2282.
  9. Sklepkiewicz, P. et al. (2015) Circ. Heart Fail. 8:362.
  10. Barandon, L. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:e80.
  11. Dufourcq, P. et al. (2002) Circ. Res. 106:3097.
  12. Yokota, T. et al. (2008) J. Immunol. 181:6061.
  13. Hausler, K.D. et al. (2004) J. Bone Miner. Res. 19:1873.
  14. Bodine, P.V.N. et al. (2004) Mol. Endocrinol. 18:1222.
  15. Wang, W.-H. et al. (2008) J. Clin. Invest. 118:1056.
Long Name
Secreted Frizzled Related Protein 1
Entrez Gene IDs
6422 (Human); 20377 (Mouse); 84402 (Rat)
Alternate Names
FRP1; FRP-1FrzA; FRPSARP-2; FrzA; SARP-2; SARP2sFRP-1; Secreted apoptosis-related protein 2; secreted frizzled-related protein 1; sFRP1; sFRP-1

Citations for Recombinant Mouse sFRP-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Vcam1+ Fibro-adipogenic Progenitors Mark Fatty Infiltration in Chronic Limb Threatening Ischemia
    Authors: Dai, Q;Wan, C;Xu, Y;Fei, K;Olivere, LA;Garrett, B;Akers, L;Peters, D;Otto, J;Kontos, CD;Ji, Z;Diao, Y;Southerland, KW;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Comparative roadmaps of reprogramming and oncogenic transformation identify Bcl11b and Atoh8 as broad regulators of cellular plasticity
    Authors: A Huyghe, G Furlan, J Schroeder, E Cascales, A Trajkova, M Ruel, F Stüder, M Larcombe, YB Yang Sun, F Mugnier, L De Matteo, A Baygin, J Wang, Y Yu, N Rama, B Gibert, J Kielbassa, L Tonon, P Wajda, N Gadot, M Brevet, M Siouda, P Mulligan, R Dante, P Liu, H Gronemeyer, M Mendoza-Pa, JM Polo, F Lavial
    Nature Cell Biology, 2022-09-08;24(9):1350-1363.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Stromal changes in the aged lung induce an emergence from melanoma dormancy
    Authors: ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna
    Nature, 2022-06-01;606(7913):396-405.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  4. Quantitative modeling of regular retinal microglia distribution
    Authors: Y Endo, D Asanuma, S Namiki, K Sugihara, K Hirose, A Uemura, Y Kubota, T Miura
    Scientific Reports, 2021-11-22;11(1):22671.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  5. Shaking culture enhances chondrogenic differentiation of mouse induced pluripotent stem cell constructs
    Authors: P Limraksasi, Y Kosaka, M Zhang, N Horie, T Kondo, H Okawa, M Yamada, H Egusa
    Sci Rep, 2020-09-14;10(1):14996.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells
    Authors: W Liang, P Han, EH Kim, J Mak, R Zhang, AG Torrente, JI Goldhaber, E Marbán, HC Cho
    Stem Cells, 2019-12-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. GSK3-activated STAT5 regulates expression of SFRPs to modulate adipogenesis
    Authors: L Wang, Y Wang, Y Meng, C Zhang, L Di
    FASEB J., 2018-03-26;0(0):fj201701314R.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
    Authors: K Saito-Diaz, H Benchabane, A Tiwari, A Tian, B Li, JJ Thompson, AS Hyde, LM Sawyer, JN Jodoin, E Santos, LA Lee, RJ Coffey, RD Beauchamp, CS Williams, AK Kenworthy, DJ Robbins, Y Ahmed, E Lee
    Dev. Cell, 2018-03-12;44(5):566-581.e8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Tbx3-dependent amplifying stem cell progeny drives interfollicular epidermal expansion during pregnancy and regeneration
    Authors: R Ichijo, H Kobayashi, S Yoneda, Y Iizuka, H Kubo, S Matsumura, S Kitano, H Miyachi, T Honda, F Toyoshima
    Nat Commun, 2017-09-11;8(1):508.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Recombinant Wnt3a and Wnt5a elicit macrophage cytokine production and tolerization to microbial stimulation via Toll-like receptor 4.
    Authors: Yu C, Nguyen T, Irvine K, Sweet M, Frazer I, Blumenthal A
    Eur J Immunol, 2014-03-07;44(5):1480-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Integration of multiple signaling regulates through apoptosis the differential osteogenic potential of neural crest-derived and mesoderm-derived Osteoblasts.
    Authors: Li S, Meyer N, Quarto N, Longaker M
    PLoS ONE, 2013-03-25;8(3):e58610.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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