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Recombinant Mouse sFRP-5 Protein, CF

Catalog #: 7195-SF
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7195-SF-050
Recombinant Mouse sFRP‑5 Protein Bioactivity.
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Product Details
Citations (3)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse sFRP-5 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.600‑7.20 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse sFRP-5 protein
Ala22-His314, with an N-terminal HA (YPYDVPDYA) tag
Accession #
NP_061250
N-terminal Sequence
Analysis
Tyr (N-terminus of Hemagglutinin tag)
Predicted Molecular Mass
34.3 kDa
SDS-PAGE
39-42 kDa, reducing conditions

Product Datasheets

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7195-SF

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7195-SF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. 
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Mouse sFRP-5 (Catalog # 7195-SF) inhibits Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.600-7.20 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-5

Secreted Frizzled Related Protein‑5 (sFRP‑5), also known as SARP‑3, belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30‑35 kDa in size and contain an N‑terminal Frizzled‑like domain with 10 conserved cysteines and a Netrin‑like C‑terminal domain (1‑3). Mature mouse sFRP‑5 shares 96% and 99% aa sequence identity with human and rat sFRP‑2, respectively (4). During embryonic development, sFRP‑5 is expressed in the anterior visceral endoderm, neural tube, foregut epithelium, and proliferating and prehypertrophic chondrocytes (5‑8). sFRP‑5 activity is required for the development of foregut, liver, ventral pancreas, and somites (6, 7). In the adult, sFRP‑5 is expressed in the retinal pigment epithelium and pancreas (4, 9). sFRP‑5 binds and antagonizes the function of mammalian Wnt‑5a and Wnt‑11 as well as Xenopus Xwnt‑8, resulting in an inhibition of both canonical and non‑canonical Wnt signaling (4, 7, 10). sFRP‑5 down‑regulation is common in breast and gastric cancer cells and is correlated with poor prognosis (11‑13). It functions as a tumor suppressor by inhibiting epithelial‑mesenchymal transition, invasiveness, and tumorigenicity of ovarian cancer cells (14). sFRP‑5 plays an important role in maintaining glucose handling and insulin sensitivity (10). It is secreted by adipocytes and is down‑regulated in mouse models of obesity and type 2 diabetes (10).

References
  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  4. Chang, J. et al. (1999) Hum. Mol. Genet. 8:575.
  5. Leaf, I. et al. (2006) Genesis 44:573.
  6. Satoh, W. et al. (2008) Genesis 46:92.
  7. Li, Y. et al. (2008) Genes Dev. 22:3050.
  8. Witte, F. et al. (2009) Gene Expr. Patterns 9:215.
  9. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  10. Ouchi, N. et al. (2010) Science 329:454.
  11. Veeck, J. et al. (2008) Carcinogenesis 29:991.
  12. Zhao, C. et al. (2009) BMC Cancer 9:224.
  13. Ho, C.M. et al. (2010) Eur. J. Clin. Invest. 40:310.
  14. Su, H.Y. et al. (2010) Int. J. Cancer 127:555.
Long Name
Secreted Frizzled Related Protein 5
Entrez Gene IDs
6425 (Human); 54612 (Mouse)
Alternate Names
frizzled-related protein 1b; FRP1B; FRP-1b; SARP3; SARP-3; secreted apoptosis related protein 3; secreted apoptosis-related protein 3; secreted frizzled-related protein 5; sFRP5; sFRP-5

Citations for Recombinant Mouse sFRP-5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells
    Authors: W Liang, P Han, EH Kim, J Mak, R Zhang, AG Torrente, JI Goldhaber, E Marbán, HC Cho
    Stem Cells, 2019-12-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Sfrp5 increases glucose-stimulated insulin secretion in the rat pancreatic beta cell line INS-1E
    Authors: M Carstensen, K Röhrig, C Niersmann, DM Ouwens, BF Belgardt, M Roden, C Herder
    PLoS ONE, 2019-03-28;14(3):e0213650.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Recombinant Wnt3a and Wnt5a elicit macrophage cytokine production and tolerization to microbial stimulation via Toll-like receptor 4.
    Authors: Yu C, Nguyen T, Irvine K, Sweet M, Frazer I, Blumenthal A
    Eur J Immunol, 2014-03-07;44(5):1480-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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