Recombinant Mouse SMOC-2 Protein, CF Summary
Product Specifications
When 5 x 104 cells/well are added to rmSMOC-2 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is 0.2‑0.8 μg/mL.
Leu30-Gly447, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6075-SM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: SMOC-2
SMOC-2 (secreted, or SPARC-related, modular calcium-binding protein 2), previously called SMAP2 (smooth muscle-associated protein 2), is a 55 kDa glycoprotein that is a member of the SPARC family of matricellular proteins (1, 2). The mouse SMOC-2 cDNA encodes 447 amino acids (aa), including a 21 aa signal sequence, a Kazal-like domain (aa 40 - 84), two thyroglobulin type-1 segments (aa 87 - 153 and 213 - 281) and two EF-hand sequences (aa 347 - 382 and 384 - 419). Of three splice variants, one shows a 13 aa substitution for aa 443 - 447, another shows an 11 aa insertion after Ala170, and a third contains both of these variations. Mature mouse SMOC-2 shares 99%, 95% and 93% aa identity with rat, human and canine SMOC-2, respectively. SMOC-2 is widely expressed in the extracellular matrix and appears to have adhesion-related functions (1 - 5). Recombinant bacterially produced human SMOC-1 and SMOC-2 can bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin, while keratinocyte SMOC-2 binds integrins alpha v beta 1 and alpha v beta 6 (3, 6). SMOC-2 promotes cell cycle progression by signaling through the integrin-linked kinase (ILK) to upregulate cyclin-D1 (4). When expressed in the endothelial extracellular matrix, it potentiates growth factor-induced angiogenesis (5). SMOC-2 expression is upregulated during neointima formation, promoting proliferation and migration of vascular smooth muscle (2). In the skin, it promotes keratinocyte attachment and migration (6). Since Kazal and thyroglobulin domains are often found in protease inhibitors, SMOC-2 is also proposed to inhibit proteases in the lung and artery (2, 7).
- Vannahme, C. et al. (2003) Biochem. J. 373:805.
- Nishimoto, S. et al. (2002) Biochim. Biophys. Acta 1576:225.
- Novinec, M. et al. (2008) Protein Expr. Purif. 62:75.
- Liu, P. et al. (2008) Mol. Biol. Cell 19:248.
- Rocnik, E.F. et al. (2006) J. Biol. Chem. 281:22855.
- Maier, S. et al. (2008) Exp. Cell Res. 314:2477.
- Wilk, J.B. et al. (2007) Am. J. Respir. Crit. Care Med. 175:554.
Citation for Recombinant Mouse SMOC-2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality
Authors: SC Schüler, JM Kirkpatric, M Schmidt, D Santinha, P Koch, S Di Sanzo, E Cirri, M Hemberg, A Ori, J von Maltza
Cell Reports, 2021-06-08;35(10):109223.
Species: Mouse
Sample Types: In Vivo, Whole Tissue
Applications: Bioassay, In Vivo
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