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Recombinant Mouse TGF-beta 2 Protein

Catalog #: 7346-B2
27 Citations 2 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
7346-B2-005/CF

With Carrier

Catalog # Availability Size / Price Qty
7346-B2-005
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (27)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TGF-beta 2 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.05‑0.3 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse TGF-beta 2 protein
Met1-Ser414
Accession #
NP_033393
N-terminal Sequence
Analysis
Ala303
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.7 kDa
SDS-PAGE
10 kDa, reducing conditions

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7346-B2 (with carrier)

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7346-B2/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7346-B2

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7346-B2/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 2

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Mouse TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 283 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 2 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature mouse TGF-beta 2 shares 100% aa identity with rat TGF-beta 2, and 97% aa identity with human, porcine, canine, equine and bovine
TGF‑ beta 2. It demonstrates cross-species activity (1). In most cells, TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII, which then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). In bone -related cells, however, TGF-beta 2 also signals through TGF-beta RIIB (a splice variant of TGF-beta RII), independently of TGF-beta  RIII (13). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

References
  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. deMartin, R. et al. (1987) EMBO J. 6:3673.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
  13. Rotzer, D. et al., (2001) EMBO J. 20:480.
Long Name
Transforming Growth Factor beta 2
Entrez Gene IDs
7042 (Human); 21808 (Mouse); 397084 (Porcine)
Alternate Names
BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2

Citations for Recombinant Mouse TGF-beta 2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Preclinical translational platform of neuroinflammatory disease biology relevant to neurodegenerative disease
    Authors: Larson, KC;Martens, LH;Marconi, M;Dejesus, C;Bruhn, S;Miller, TA;Tate, B;Levenson, JM;
    Journal of neuroinflammation
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration
    Authors: Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ;
    Cell reports
    Species: Mouse
    Sample Types:
  3. Klotho prevents transforming growth factor-?2-induced senescent-like morphological changes in the retinal pigment epithelium
    Authors: Jang, HY;Kim, SJ;Park, KS;Kim, JH;
    Cell death & disease
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. RNA binding protein DDX5 restricts RORgammat+ Treg suppressor function to promote intestine inflammation
    Authors: S Ma, Q Yang, N Chen, A Zheng, N Abbasi, G Wang, PR Patel, BS Cho, BA Yee, L Zhang, H Chu, SM Evans, GW Yeo, Y Zheng, WJM Huang
    Science Advances, 2023-02-01;9(5):eadd6165.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Long Non-Coding RNA H19 Prevents Lens Fibrosis through Maintaining Lens Epithelial Cell Phenotypes
    Authors: L Xiong, Y Sun, J Huang, P Ma, X Wang, J Wang, B Chen, J Chen, M Huang, S Huang, Y Liu
    Cells, 2022-08-17;11(16):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells
    Authors: M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu
    Journal of Neuroinflammation, 2022-07-14;19(1):182.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Follistatin promotes LIN28B-mediated supporting cell reprogramming and hair cell regeneration in the murine cochlea
    Authors: XJ Li, C Morgan, LA Goff, A Doetzlhofe
    Science Advances, 2022-02-11;8(6):eabj7651.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. A shared transcriptional code orchestrates temporal patterning of the central nervous system
    Authors: A Sagner, I Zhang, T Watson, J Lazaro, M Melchionda, J Briscoe
    PloS Biology, 2021-11-12;19(11):e3001450.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Mechanical forces regulate endothelial-to-mesenchymal transition and atherosclerosis via an Alk5-Shc mechanotransduction pathway
    Authors: V Mehta, KL Pang, CS Givens, Z Chen, J Huang, DT Sweet, H Jo, JS Reader, E Tzima
    Science Advances, 2021-07-09;7(28):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Type II Collagen-Specific B Cells Induce Immune Tolerance in Th1-Skewed, Th2-Skewed, and Arthritis-Prone Strains of Mice
    Authors: SM Farooq, HM Ashour
    Cells, 2021-04-12;10(4):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Microglial identity and inflammatory responses are controlled by the combined effects of neurons and astrocytes
    Authors: PS Baxter, O Dando, K Emelianova, X He, S McKay, GE Hardingham, J Qiu
    Cell Reports, 2021-03-23;34(12):108882.
    Species: Human, Mouse, Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  12. TGF&beta signaling curbs cell fusion and muscle regeneration
    Authors: F Girardi, A Taleb, M Ebrahimi, A Datye, DG Gamage, C Peccate, L Giordani, DP Millay, PM Gilbert, B Cadot, F Le Grand
    Nature Communications, 2021-02-02;12(1):750.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  13. Interferon Gamma Mediates Hematopoietic Stem Cell Activation and Niche Relocalization through BST2
    Authors: MA Florez, KA Matatall, Y Jeong, L Ortinau, PW Shafer, AM Lynch, R Jaksik, M Kimmel, D Park, KY King
    Cell Reports, 2020-12-22;33(12):108530.
    Species: Mouse, Transgenic Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. Disruption of fibronectin fibrillogenesis affects intraocular pressure (IOP) in BALB/cJ mice
    Authors: JA Faralli, MS Filla, CM McDowell, DM Peters
    PLoS ONE, 2020-08-21;15(8):e0237932.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. Pharmacological Targeting of BET Bromodomains Inhibits Lens Fibrosis via Downregulation of MYC Expression
    Authors: X Wang, B Wang, N Zhao, C Wang, M Huang, B Chen, J Chen, Y Sun, L Xiong, S Huang, Y Liu
    Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4748-4758.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Tissue Culture
  16. miR-486 is modulated by stretch and increases ventricular growth
    Authors: S Lange, I Banerjee, K Carrion, R Serrano, L Habich, R Kameny, L Lengenfeld, N Dalton, R Meili, E Börgeson, K Peterson, M Ricci, J Lincoln, M Ghassemian, JR Fineman, JC Del Álamo, V Nigam
    JCI Insight, 2019-09-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Activation of the Regulatory T-Cell/Indoleamine 2,3-Dioxygenase Axis Reduces Vascular Inflammation and Atherosclerosis in Hyperlipidemic Mice.
    Authors: Maria J Forteza, Konstantinos A Polyzos, Roland Baumgartn, Bianca E Suur, Marion Mussbache, Daniel K Johansson, Andreas Hermansso, Göran K Hansson, Daniel F J Ketelhuth
    Frontiers in Immunology, 2018-05-07;0(0):1664-3224.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Aldose reductase modulates acute activation of mesenchymal markers via the ?-catenin pathway during cardiac ischemia-reperfusion
    Authors: D Thiagaraja, K O' Shea, G Sreejit, R Ananthakri, N Quadri, Q Li, AM Schmidt, K Gabbay, R Ramasamy
    PLoS ONE, 2017-11-30;12(11):e0188981.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Interleukin-17A-promoted MSC2 polarization related with new bone formation of ankylosing spondylitis
    Authors: T He, Y Huang, C Zhang, D Liu, C Cheng, W Xu, X Zhang
    Oncotarget, 2017-09-11;8(57):96993-97008.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Bone-induced expression of integrin ?3 enables targeted nanotherapy of breast cancer metastases
    Authors: MH Ross, AK Esser, GC Fox, AH Schmieder, X Yang, G Hu, D Pan, X Su, Y Xu, DV Novack, T Walsh, GA Colditz, GH Lukaszewic, E Cordell, JS Novack, JAJ Fitzpatric, DL Waning, KS Mohammad, TA Guise, GM Lanza, KN Weilbaeche
    Cancer Res., 2017-08-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Therapeutic effect of a novel histone deacetylase 6 inhibitor, CKD-L, on collagen-induced arthritis in vivo and regulatory T cells in rheumatoid arthritis in vitro
    Authors: BR Oh, DH Suh, D Bae, N Ha, YI Choi, HJ Yoo, JK Park, EY Lee, EB Lee, YW Song
    Arthritis Res. Ther., 2017-07-03;19(1):154.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Cell-Type-Specific Chromatin States Differentially Prime Squamous Cell Carcinoma Tumor-Initiating Cells for Epithelial to Mesenchymal Transition
    Authors: Cédric Blanpain
    Cell Stem Cell, 2016-11-23;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.
    Authors: Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K
    Nat Commun, 2015-02-19;6(0):6329.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Myocardin-related transcription factors control the motility of epicardium-derived cells and the maturation of coronary vessels.
    Authors: Trembley M, Velasquez L, de Mesy Bentley K, Small E
    Development, 2015-01-01;142(1):21-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Impairment of endothelial-mesenchymal transformation during atrioventricular cushion formation in Tmem100 null embryos.
    Authors: Mizuta K, Sakabe M, Hashimoto A, Ioka T, Sakai C, Okumura K, Hattammaru M, Fujita M, Araki M, Somekawa S, Saito Y, Nakagawa O
    Dev Dyn, 2014-11-07;244(1):31-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Matrix metalloproteinase-9-null mice are resistant to TGF-beta-induced anterior subcapsular cataract formation.
    Authors: Korol A, Pino G, Dwivedi D, Robertson J, Deschamps P, West-Mays J
    Am J Pathol, 2014-05-06;184(7):2001-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Ex Vivo
  27. Factors expressed by murine embryonic pancreatic mesenchyme enhance generation of insulin-producing cells from hESCs.
    Authors: Guo T, Landsman L, Li N, Hebrok M
    Diabetes, 2013-01-10;62(5):1581-92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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Recombinant Mouse TGF-beta 2 Protein
By Takeshi Saito on 12/02/2020
Application: In vitro bioactivity in cell culture
Recombinant Mouse TGF-beta 2 Protein
By Anonymous on 03/28/2019
Application: direct ELISA

I coated my plates with 50ng/well of recombinant mouse TGFbeta 2 proteins and in-house produced human TGF beta 3. I wanted to check the specificity of Abcam's TGF beta 3 antibody. I did not use it because it showed cross reactivity with TGF beta 2

Other Recombinant Mouse TGF-beta 2 Protein 7346-B2