Recombinant Mouse TLR4 Fc Chimera Protein, CF

Catalog #: 9149-TR
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9149-TR-050

Recombinant Mouse TLR4 Fc Chimera Protein Bioactivity

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Product Details

Citations (3)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TLR4 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse TLR4 Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human MD-2 (Catalog # 1787-MD) that produces 50% of the optimal binding response is approximately 0.15-0.9 μg/mL

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TLR4 protein

Mouse TLR4 (Asn26-Lys629) Accession # Q9QUK6	DIEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9QUK6

N-terminal Sequence
Analysis

Asn26

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

96 kDa

SDS-PAGE

115-137 kDa, reducing conditions

Product Datasheets

9149-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9149-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Mouse TLR4 Fc Chimera (Catalog # 9149-TR) is coated at 2 µg/mL (100 µL/well), Recombinant Human MD-2 (Catalog # 1787-MD) binds with a typical ED₅₀ of 0.15-0.9 µg/mL.

Reconstitution Calculator

Background: TLR4

TLR4, also known as CD284, is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. The complex of TLR4 with MD-2 functions as a critical receptor for bacterial endotoxin/lipopolysaccharide (LPS) (1-3). Mature mouse TLR4 consists of a 613 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 176 aa cytoplasmic domain. TLR4 contains 19 leucine rich repeats in its ECD and one cytoplasmic Toll/IL-1 receptor (TIR) domain (4-6). Within the ECD, mouse TLR4 shares 63% and 83% aa sequence identity with human and rat TLR4, respectively. On monocytes, macrophages, dendritic cells, and B cells, MD‑2 expression is required for cell surface localization of TLR4 and for optimal LPS‑induced TLR4 signaling (7-10). MD‑2 also forms soluble disulfide-linked homo-oligomers which can interact with TLR4 (8). Through a domain separate from its TLR4-binding domain, MD‑2 extracts LPS from circulating CD14‑LPS complexes and carries the LPS into a ternary complex with TLR4 (11-13). The interaction of
MD‑2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (2, 14). Increased levels of plasma MD‑2 in septic shock patients sensitizes MD‑2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (15).

References

Mukherjee, S. et al. (2016) Braz. J. Infect. Dis. 20:193.
Plociennikowska, A. et al. (2015) Cell. Mol. Life Sci. 72:557.
Erridge, C. (2010) J. Leukoc. Biol. 87:989.
Medzhitov, R. et al. (1997) Nature 388:394.
Poltorak, A. et al. (1998) Science 282:2085.
Qureshi, S.T. et al. (1999) J. Exp. Med. 189:615.
Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12156.
Akashi, S. et al. (2000) J. Immunol. 164:3471.
Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4186.
Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
Pugin, J. et al. (2004) Blood 104:4071.

Long Name

Toll-like Receptor 4

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Alternate Names

ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

Related Research Areas

Citations for Recombinant Mouse TLR4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Structural elucidation and immunomodulatory activities in vitro of type I and II arabinogalactans from different origins of Astragalus membranaceus
Authors: Wang, K;Zhou, Y;Li, M;Chen, Z;Wu, Z;Ji, W;Wang, J;Zhang, Y;
Carbohydrate polymers
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay
Serum Amyloid A1 Exacerbates Hepatic Steatosis via TLR4 Mediated NF-kappaB Signaling Pathway
Authors: B Jiang, D Wang, Y Hu, W Li, F Liu, X Zhu, X Li, H Zhang, H Bai, Q Yang, X Yang, J Ben, Q Chen
Molecular Metabolism, 2022-03-03;0(0):101462.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Surface Plasmon Resonance (SPR
Immunomodulatory effects of xanthan gum in LPS-stimulated RAW 264.7 macrophages
Authors: F Liu, X Zhang, P Ling, J Liao, M Zhao, L Mei, H Shao, P Jiang, Z Song, Q Chen, F Wang
Carbohydr Polym, 2017-04-03;169(0):65-74.
Applications: Bioassay