Recombinant Mouse TLR7 Protein, CF

Catalog #: 9568-TR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9568-TR-050

Product Details

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TLR7 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

E. coli-derived mouse TLR7 protein
Asn275-Phe444, with an N-terminal Met and a C-terminal 6-His tag

Accession #

P58681

N-terminal Sequence
Analysis

Met and Met313

Predicted Molecular Mass

21 kDa

SDS-PAGE

16 kDa and 21 kDa, reducing conditions

Product Datasheets

9568-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9568-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 200 μg/mL in 10 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TLR7

TLR7 (Toll-like receptor 7) is a member of the Toll-like receptor family of innate immune receptors. It is mainly expressed in lung, placenta and spleen and belongs to a new sub-family of the TLRs comprising of TLR7, TLR8, an TLR9 (1, 2). Mouse TLR7 cDNA encodes a 1050 amino acid (aa) precursor that contains a 26 aa signal sequence, an 811 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 192 aa cytoplasmic domain. Mouse TLR7 is 81% aa identical to mature human TLR7. TLR7 is located in the endosomes and involved in the sequence-dependent sensing of single-stranded RNA, thereby detecting bacterial or viral nucleic acids (3, 4, 5, 6).

References