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Recombinant Mouse TSLP Protein

Catalog #: 555-TS
33 Citations 6 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
555-TS-010/CF

With Carrier

Catalog # Availability Size / Price Qty
555-TS-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (33)
FAQs
Reviews (6)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TSLP Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse IL-7 R alpha. The ED50 for this effect is 0.5-2.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse TSLP protein
Tyr20-Glu140, with a C-terminal 10-His tag
Accession #
NP_067342
N-terminal Sequence
Analysis
Tyr20
Predicted Molecular Mass
15 kDa
SDS-PAGE
22-29 kDa, reducing conditions

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555-TS (with carrier)

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SDS

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555-TS/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

555-TS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

555-TS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. Within the mature region, there are three potential N-glycosylation sites and 7 cysteine residues. Mouse TSLP shares approximately 43% aa sequence identity with human TSLP. The gene for mouse TSLP has been localized to chromosome 18. By Northern and RT-PCR analysis, mouse TSLP expression has been detected in spleen, thymus, kidney, lung and bone marrow. TSLP is proposed to signal through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a new member of the hemopoietin receptor family most closely related to R gamma c.

References
  1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
  2. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  3. Pandey, A. et al. (2000) Nature Immunol. 1:59.
  4. Reche, P.A. et al. (2001) J. Immunol. 167:336.
Long Name
Thymic Stromal Lymphopoietin
Entrez Gene IDs
85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)
Alternate Names
thymic stromal lymphopoietin; TSLP

Citations for Recombinant Mouse TSLP Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity
    Authors: Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Crosstalk between ILC2s and Th2 cells varies among mouse models
    Authors: RK Gurram, D Wei, Q Yu, MJ Butcher, X Chen, K Cui, G Hu, M Zheng, X Zhu, J Oh, B Sun, JF Urban, K Zhao, WJ Leonard, J Zhu
    Cell Reports, 2023-02-02;42(2):112073.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Context-dependent function of TSLP and IL-1beta in skin allergic sensitization and atopic march
    Authors: J Segaud, W Yao, P Marschall, F Daubeuf, C Lehalle, B German, P Meyer, P Hener, C Hugel, E Flatter, M Guivarch, L Clauss, SF Martin, M Oulad-Abde, M Li
    Nature Communications, 2022-09-01;13(1):4703.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Interleukin-33 and thymic stromal lymphopoietin, but not interleukin-25, are crucial for development of airway eosinophilia induced by chitin
    Authors: K Arae, M Ikutani, K Horiguchi, S Yamaguchi, Y Okada, H Sugiyama, K Orimo, H Morita, H Suto, K Okumura, H Taguchi, K Matsumoto, H Saito, K Sudo, S Nakae
    Scientific Reports, 2021-03-15;11(1):5913.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Thymic stromal lymphopoietin limits primary and recall CD8+ T-cell anti-viral responses
    Authors: R Ebina-Shib, EE West, R Spolski, P Li, J Oh, M Kazemian, D Gromer, P Swanson, N Du, DB McGavern, WJ Leonard
    Elife, 2021-01-13;10(0):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. AMPK induces regulatory innate lymphoid cells after traumatic brain injury
    Authors: B Baban, M Braun, H Khodadadi, A Ward, K Alverson, A Malik, K Nguyen, S Nazarian, DC Hess, S Forseen, AF Post, FL Vale, JR Vender, MN Hoda, O Akbari, K Vaibhav, KM Dhandapani
    JCI Insight, 2021-01-11;6(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Yupingfeng Granule Improves Th2-Biased Immune State in Microenvironment of Hepatocellular Carcinoma through TSLP-DC-OX40L Pathway
    Authors: F Yao, Q Yuan, X Song, L Zhou, G Liang, G Jiang, L Zhang
    Evid Based Complement Alternat Med, 2020-04-09;2020(0):1263053.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch
    Authors: SK Mishra, JJ Wheeler, S Pitake, H Ding, C Jiang, T Fukuyama, JS Paps, P Ralph, J Coyne, M Parkington, J DeBrecht, LC Ehrhardt-H, GP Cruse, W Bäumer, RR Ji, MC Ko, T Olivry
    Cell Rep, 2020-04-07;31(1):107472.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. TSLP protects against liver I/R injury via activation of the PI3K/Akt pathway
    Authors: S Li, Z Yi, M Deng, MJ Scott, C Yang, W Li, Z Lei, NM Santerre, P Loughran, TR Billiar
    JCI Insight, 2019-11-14;4(22):.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Bioassay
  10. Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis
    Authors: E Ragonnaud, K Moritoh, M Bodogai, F Gusev, S Garaud, C Chen, X Wang, T Baljinnyam, KG Becker, RW Maul, K Willard-Ga, E Rogaev, A Biragyn
    Cancer Res., 2019-10-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. TSLP Exacerbates Septic Inflammation via Murine Double Minute 2 (MDM2) Signaling Pathway
    Authors: NR Han, PD Moon, HM Kim, HJ Jeong
    J Clin Med, 2019-09-01;8(9):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. Statins can suppress dendritic cell-mediated Th2 responses through the repression of OX40-ligand and CCL17 expression
    Authors: N Inagaki-Ka, T Ito, M Inaba, Y Azuma, A Tanaka, PTX Vien, K Kibata, A Satake, S Nomura
    Eur. J. Immunol., 2019-07-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. c-Kit-positive ILC2s exhibit an ILC3-like signature that may contribute to IL-17-mediated pathologies
    Authors: JH Bernink, Y Ohne, MBM Teunissen, J Wang, J Wu, L Krabbendam, C Guntermann, R Volckmann, J Koster, S van Tol, I Ramirez, Y Shrestha, MA de Rie, H Spits, X Romero Ros, AA Humbles
    Nat. Immunol., 2019-07-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  14. The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice
    Authors: M Han, C Rajput, JY Hong, J Lei, JL Hinde, Q Wu, JK Bentley, MB Hershenson
    J. Immunol., 2017-07-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. IL-7R? Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin
    Authors: L Kummola, Z Ortutay, X Chen, S Caucheteux, S Hämäläinen, S Aittomäki, R Yagi, J Zhu, M Pesu, WE Paul, IS Junttila
    J. Immunol., 2017-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Innate immunological function of TH2 cells in vivo.
    Authors: Guo L, Huang Y, Chen X, Hu-Li J, Urban J, Paul W
    Nat Immunol, 2015-08-31;16(10):1051-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Comparative genomics reveals multistep pathogenesis of E2A-PBX1 acute lymphoblastic leukemia.
    Authors: Duque-Afonso J, Feng J, Scherer F, Lin C, Wong S, Wang Z, Iwasaki M, Cleary M
    J Clin Invest, 2015-08-24;125(9):3667-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. CD326(lo)CD103(lo)CD11b(lo) dermal dendritic cells are activated by thymic stromal lymphopoietin during contact sensitization in mice.
    Authors: Ochiai S, Roediger B, Abtin A, Shklovskaya E, Fazekas de St Groth B, Yamane H, Weninger W, Le Gros G, Ronchese F
    J Immunol, 2014-07-23;193(5):2504-11.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  19. Control of T helper 2 responses by transcription factor IRF4-dependent dendritic cells.
    Authors: Gao Y, Nish S, Jiang R, Hou L, Licona-Limon P, Weinstein J, Zhao H, Medzhitov R
    Immunity, 2013-09-26;39(4):722-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. A dynamic dual role of IL-2 signaling in the two-step differentiation process of adaptive regulatory T cells.
    Authors: Guo Z, Khattar M, Schroder P, Miyahara Y, Wang G, He X, Chen W, Stepkowski S
    J Immunol, 2013-02-20;190(7):3153-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, Is crucial for murine asthma.
    Authors: Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Korner H, Cua D, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide D, Matsumoto K, Nakae S
    J Immunol, 2012-08-31;189(7):3641-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Preclinical Evaluation of Local JAK1 and JAK2 Inhibition in Cutaneous Inflammation.
    Authors: Fridman JS, Scherle PA, Collins R, Burn T, Neilan CL, Hertel D, Contel N, Haley P, Thomas B, Shi J, Collier P, Rodgers JD, Shepard S, Metcalf B, Hollis G, Newton RC, Yeleswaram S, Friedman SM, Vaddi K
    J. Invest. Dermatol., 2011-06-16;131(9):1838-44.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. IL-13 induces skin fibrosis in atopic dermatitis by thymic stromal lymphopoietin.
    Authors: Oh MH, Oh SY, Yu J
    J. Immunol., 2011-05-16;186(12):7232-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Peroxisome proliferator-activated receptor gamma-mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC/Tnd mice.
    Authors: Jung K, Tanaka A, Fujita H, Matsuda A, Oida K, Karasawa K, Okamoto N, Ohmori K, Jee Y, Shin T, Matsuda H
    J. Allergy Clin. Immunol., 2011-01-05;127(2):420-29.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.
    Authors: Perrigoue JG, Saenz SA, Siracusa MC, Allenspach EJ, Taylor BC, Giacomin PR, Nair MG, Du Y, Zaph C, Van Rooijen N, Comeau MR, Pearce EJ, Laufer TM, Artis D
    Nat. Immunol., 2009-05-24;10(7):697-705.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  26. Divergent functions for airway epithelial matrix metalloproteinase 7 and retinoic acid in experimental asthma.
    Authors: Goswami S, Angkasekwinai P, Shan M, Greenlee KJ, Barranco WT, Polikepahad S, Seryshev A, Song LZ, Redding D, Singh B, Sur S, Woodruff P, Dong C, Corry DB, Kheradmand F
    Nat. Immunol., 2009-03-29;10(5):496-503.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  27. TSLP conditions the lung immune environment for the generation of pathogenic innate and antigen-specific adaptive immune responses.
    Authors: Headley MB, Zhou B, Shih WX, Aye T, Comeau MR, Ziegler SF
    J. Immunol., 2009-02-01;182(3):1641-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  28. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
    Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
    Arthritis Rheum., 2008-11-01;58(11):3510-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development.
    Authors: Vang KB, Yang J, Mahmud SA, Burchill MA, Vegoe AL, Farrar MA
    J. Immunol., 2008-09-01;181(5):3285-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Thymic stromal lymphopoietin and thymic stromal lymphopoietin-conditioned dendritic cells induce regulatory T-cell differentiation and protection of NOD mice against diabetes.
    Authors: Besin G, Gaudreau S, Menard M, Guindi C, Dupuis G, Amrani A
    Diabetes, 2008-05-13;57(8):2107-17.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  31. A mechanism for the initiation of allergen-induced T helper type 2 responses.
    Authors: Sokol CL, Barton GM, Farr AG, Medzhitov R
    Nat. Immunol., 2008-03-01;9(3):310-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
    Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
    J. Clin. Invest., 2007-12-01;117(12):3868-3878.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  33. Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis.
    Authors: Osborn MJ, Ryan PL, Kirchhof N, Panoskaltsis-Mortari A, Mortari F, Tudor KS
    Blood, 2003-09-25;103(3):843-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Is Recombinant Mouse TSLP Protein (Catalog # 555-TS) the short or long isoform?

    • Mouse TSLP is considered to be an ortholog of human long TSLP (Fornasa et al., 2015). However, TSLP variants in mice are currently uncertain as a murine short TSLP variant has not been described or annotated in Reference Sequence so far (Bjerkan et al., 2016).

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Reviews for Recombinant Mouse TSLP Protein

Average Rating: 3.8 (Based on 6 Reviews)

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