Recombinant Mouse VSIG2 Fc Chimera Protein, CF

Catalog #: 9598-VS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9598-VS-050

Recombinant Mouse VSIG2 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse VSIG2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 0.75-4.5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse VSIG2 protein

Mouse VSIG2 (Val25-Ala244) Accession # Q9Z109-1	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9Z109-1

N-terminal Sequence
Analysis

Val25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa

SDS-PAGE

58-66 kDa, reducing conditions

Product Datasheets

9598-VS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9598-VS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse VSIG2 Fc Chimera (Catalog # 9598‑VS) inhibits IL-17 secretion by human peripheral blood mononuclear cells (PBMC), in the presence of anti-CD3 antibody. The ED₅₀ for this effect is 0.75-4.5 μg/mL.

Reconstitution Calculator

Background: VSIG2

V-set and immunoglobulin domain-containing protein 2 (VSIG2), also known as CTM or CT-like protein, is a member of the much larger Ig superfamily. Mature mouse VSIG2 is a single-pass type I transmembrane protein with a 220 aa extracellular domain (ECD) and a 63 aa cytoplasmic region linked by a short transmembrane domain. The ECD of VSIG2 contains one Ig-like C2-type (immunoglobulin-like) and one Ig-like V-type (immunoglobulin-like) domain. Mouse VSIG2 ECD shares 85% and 94% identity with human and rat, respectively. In humans, VSIG2 is highly expressed in stomach, colon, prostate, trachea and thyroid glands and weakly in bladder and lung (1). V-set domains are Ig-like domains resembling the antibody variable domain and are found in diverse protein families from immunoglobulin light and heavy chains to T cell receptors and adhesion molecules (2). Several VSIG molecules, including VSIG1 and VSIG4, can be classified into the junctional adhesion molecule (JAM) family based on the immunoglobulin folds, V‐ and C2‐types, found in the ECD (3, 4). While the function of VSIG2 is currently unknown, based on the structural similarity to JAM molecules, it is most likely a cell adhesion molecule (2-4). R&D Systems in-house testing indicates that VSIG2 inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC.

Long Name

V-Set and Immunoglobulin Domain Containing 2

Entrez Gene IDs

23584 (Human); 57276 (Mouse); 300520 (Rat)

Alternate Names

2210413P10Rik; Cortical thymocyte-like protein; CTH; CTHCTXLcortical thymocyte receptor (X. laevis CTX) like; CT-like protein; CTXL; V-set and immunoglobulin domain containing 2; V-set and immunoglobulin domain-containing protein 2; VSIG2