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Recombinant Mouse Wnt-2b Protein, CF

Catalog #: 3900-WN/CF
3 Citations Datasheet / COA / SDS

Discontinued Product

3900-WN/CF has been discontinued.
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Wnt-2b Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining, under reducing conditions
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit cell proliferation/survival assay using C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1-5 µg/ml.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-2b protein
Ser57-Thr389
Accession #
AAC25397
N-terminal Sequence
Analysis
Ser57 & Asp71
Predicted Molecular Mass
38 kDa
SDS-PAGE
41-60 kDa, reducing conditions

Product Datasheets

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3900-WN/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3900-WN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-2b

Mouse Wnt-2b (also known as Wnt-13) is a member of the large and highly conserved Wnt family of signaling molecules that have roles in pattern formation, cell fate decision, axon guidance, and tumor formation (1). Mouse Wnt-2b is 389 amino acids (aa) in length and includes a signal peptide (2). Mature mouse Wnt-2b has a predicted molecular mass of 38 kDa and shares 96% aa sequence identity with the human protein. Wnt-2b initiates B-catenin-mediated canonical signaling and is implicated in the development of the lung, kidney, liver, and eye, as well as being present in ovarian surface epithelial cells (3-8). Pathological expression of Wnt-2b in tumors is correlated with increased cell proliferation and decreased survival (9).

References
  1. Anastas, J.N. and R.T. Moon (2013) Nat. Rev. Cancer 13:11.
  2. Zakin, L.D. et al. (1998) Mech. Dev. 73:107.
  3. Goss, A.M. et al. (2009) Dev. Cell 17:290.
  4. Ricken, A. et al. (2002) Endocrinology 143:2741.
  5. Lin, Y. et al. (2001) Dev. Dyn. 222:26.
  6. Jasoni, C. et al. (1999) Dev. Dyn. 215:215.
  7. Ober, E.A. et al. (2006) Nature 442:688.
  8. Kubo, F. et al. (2005) Development 132:2759.
  9. Kobayashi, M. et al. (2012) Exp. Ther. Med. 3:952.
Long Name
Wingless-type MMTV Integration Site Family Member 2b
Entrez Gene IDs
7482 (Human); 22414 (Mouse); 116466 (Rat)
Alternate Names
Protein Wnt-13; protein Wnt-2b; wingless-type MMTV integration site family, member 2B; Wnt-13; WNT13XWNT2, Xenopus, homolog of; Wnt2b; Wnt-2b; XWNT2; XWNT2wingless-type MMTV integration site family, member 13

Citations for Recombinant Mouse Wnt-2b Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. WRN conditioned media is sufficient for in vitro propagation of intestinal organoids from large farm and small companion animals
    Authors: RH Powell, MS Behnke
    Biol Open, 2017-05-15;0(0):.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  2. Atf3 mutant mice show reduced axon regeneration and impaired regeneration-associated gene induction after peripheral nerve injury
    Open Biol, 2016-08-01;6(8):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Canonical wnt signaling in dendritic cells regulates Th1/Th17 responses and suppresses autoimmune neuroinflammation.
    Authors: Suryawanshi A, Manoharan I, Hong Y, Swafford D, Majumdar T, Taketo M, Manicassamy B, Koni P, Thangaraju M, Sun Z, Mellor A, Munn D, Manicassamy S
    J Immunol, 2015-02-20;194(7):3295-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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