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Recombinant Mouse Wnt-2b Protein

Catalog #: 3900-WN
3 Citations Datasheet / COA / SDS

Discontinued Product

3900-WN has been discontinued.
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Wnt-2b Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining, under reducing conditions
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit cell proliferation/survival assay using C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1-5 µg/ml.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-2b protein
Ser57-Thr389
Accession #
AAC25397
N-terminal Sequence
Analysis
Ser57 & Asp71
Predicted Molecular Mass
38 kDa
SDS-PAGE
41-60 kDa, reducing conditions

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3900-WN (with carrier)

Product Datasheet
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3900-WN/CF (carrier free)

Discontinued Product

3900-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-2b

Mouse Wnt-2b (also known as Wnt-13) is a member of the large and highly conserved Wnt family of signaling molecules that have roles in pattern formation, cell fate decision, axon guidance, and tumor formation (1). Mouse Wnt-2b is 389 amino acids (aa) in length and includes a signal peptide (2). Mature mouse Wnt-2b has a predicted molecular mass of 38 kDa and shares 96% aa sequence identity with the human protein. Wnt-2b initiates B-catenin-mediated canonical signaling and is implicated in the development of the lung, kidney, liver, and eye, as well as being present in ovarian surface epithelial cells (3-8). Pathological expression of Wnt-2b in tumors is correlated with increased cell proliferation and decreased survival (9).

References
  1. Anastas, J.N. and R.T. Moon (2013) Nat. Rev. Cancer 13:11.
  2. Zakin, L.D. et al. (1998) Mech. Dev. 73:107.
  3. Goss, A.M. et al. (2009) Dev. Cell 17:290.
  4. Ricken, A. et al. (2002) Endocrinology 143:2741.
  5. Lin, Y. et al. (2001) Dev. Dyn. 222:26.
  6. Jasoni, C. et al. (1999) Dev. Dyn. 215:215.
  7. Ober, E.A. et al. (2006) Nature 442:688.
  8. Kubo, F. et al. (2005) Development 132:2759.
  9. Kobayashi, M. et al. (2012) Exp. Ther. Med. 3:952.
Long Name
Wingless-type MMTV Integration Site Family Member 2b
Entrez Gene IDs
7482 (Human); 22414 (Mouse); 116466 (Rat)
Alternate Names
Protein Wnt-13; protein Wnt-2b; wingless-type MMTV integration site family, member 2B; Wnt-13; WNT13XWNT2, Xenopus, homolog of; Wnt2b; Wnt-2b; XWNT2; XWNT2wingless-type MMTV integration site family, member 13

Citations for Recombinant Mouse Wnt-2b Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. WRN conditioned media is sufficient for in vitro propagation of intestinal organoids from large farm and small companion animals
    Authors: RH Powell, MS Behnke
    Biol Open, 2017-05-15;0(0):.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  2. Atf3 mutant mice show reduced axon regeneration and impaired regeneration-associated gene induction after peripheral nerve injury
    Open Biol, 2016-08-01;6(8):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Canonical wnt signaling in dendritic cells regulates Th1/Th17 responses and suppresses autoimmune neuroinflammation.
    Authors: Suryawanshi A, Manoharan I, Hong Y, Swafford D, Majumdar T, Taketo M, Manicassamy B, Koni P, Thangaraju M, Sun Z, Mellor A, Munn D, Manicassamy S
    J Immunol, 2015-02-20;194(7):3295-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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