Recombinant Porcine IFN-alpha (Mammalian) Protein Summary
Product Specifications
Interferon was titrated with the use of the cytopathic effect inhibition assay as listed
Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37 (2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.
The units are determined by use of a Human IFN Alpha A (Hu IFN-alpha A [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A (Hu IFN-alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257(1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
cDNA obtained from porcine leukocyte mRNA expressed in mammalian cells.
Accession # NM_214393.1
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
17105-1
| Formulation | Phosphate buffered saline with 0.1% bovine serum albumin (BSA) |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Reconstitution Calculator
Background: IFN-alpha
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Citations for Recombinant Porcine IFN-alpha (Mammalian) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Porcine HERC6 acts as major E3 ligase for ISGylation and is auto-ISGylated for effective ISGylation in porcine
Authors: Ba, C;Tian, C;Bo, X;Xu, C;Zhang, Y;Sun, X;Nan, Y;Wu, C;
Microbial pathogenesis
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Porcine circovirus type 2 infection inhibits the activation of type I interferon signaling via capsid protein and host gC1qR
Authors: Z Wang, J Chen, QG Zhang, K Huang, D Ma, Q Du, D Tong, Y Huang
Veterinary Microbiology, 2022-01-22;266(0):109354.
Species: Porcine
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
PRRSV Vaccine Strain-Induced Secretion of Extracellular ISG15 Stimulates Porcine Alveolar Macrophage Antiviral Response against PRRSV
Authors: H Liu, B Shi, Z Zhang, B Zhao, G Zhao, Y Li, Y Nan
Viruses, 2020-09-10;12(9):.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Identification of three site mutations in nonstructural protein 1 β, glycoprotein 3 and glycoprotein 5 that correlate with increased interferon α resistance of porcine reproductive and respiratory syndrome virus
Authors: J Su, X Zhang, B He, X Ge, J Han, L Zhou, X Guo, H Yang
Vet. Microbiol., 2019-08-19;236(0):108395.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Characterization of the attachment and infection by Porcine reproductive and respiratory syndrome virus 1 isolates in bone marrow-derived dendritic cells
Authors: YL Li, L Darwich, E Mateu
Vet. Microbiol., 2018-08-11;223(0):181-188.
Species: Porcine
Sample Types: Cell Culture Supernates
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Genome-wide analysis of antiviral signature genes in porcine macrophages at different activation statuses.
Authors: Sang Y, Brichalli W, Rowland R, Blecha F
PLoS ONE, 2014-02-05;9(2):e87613.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Cytokine protein expression levels in tracheobronchial lymph node homogenates of pigs infected with pseudorabies virus.
Authors: Miller LC, Zanella EL, Waters WR
Clin. Vaccine Immunol., 2010-03-10;17(5):728-34.
Species: Porcine
Sample Types: N/A
Applications: ELISA (Standard)
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