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Recombinant Porcine IL-18/IL-1F4 Protein

Catalog #: 588-PL
6 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
588-PL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
588-PL-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Porcine IL-18/IL-1F4 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is 10-40 ng/mL in the presence of 20 ng/mL rhTNF-alpha.
Source
E. coli-derived porcine IL-18/IL-1F4 protein
Tyr36-Asn192
Accession #
O19073
N-terminal Sequence
Analysis
Tyr36
Predicted Molecular Mass
18.0 kDa

Product Datasheets

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588-PL (with carrier)

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588-PL/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

588-PL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

588-PL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed
IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11).
IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature porcine IL-18 shares 88% amino acid sequence identity with canine and feline IL-18 and 61%-76% with human, mouse, rat,  and rhesus IL-18 (15).

References
  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
  3. Gu, Y. et al. (1997) Science 275:206.
  4. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  5. Cheung, H. et al. (2005) J. Immunol. 174:5351.
  6. Novick, D. et al. (1999) Immunity 10:127.
  7. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
  8. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  9. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
  10. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
  11. Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
  12. Li, J. et al. (2004) Cell. Immunol. 227:103.
  13. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
  14. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
  15. Muneta, Y. et al. (2000) Cytokine 12:566.
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

Citations for Recombinant Porcine IL-18/IL-1F4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Early Immune Initiation by Porcine Cells following Toxoplasma gondii Infection versus TLR Ligation
    Authors: B Hamid, J Schlosser-, L Bechtold, F Ebner, S Rausch, S Hartmann
    Microorganisms, 2021-08-28;9(9):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Protective porcine influenza virus-specific monoclonal antibodies recognize similar haemagglutinin epitopes as humans
    Authors: B Holzer, P Rijal, A McNee, B Paudyal, V Martini, B Clark, T Manjegowda, FJ Salguero, E Bessell, JC Schwartz, K Moffat, M Pedrera, SP Graham, A Noble, M Bonnet-Di, RM La Ragione, W Mwangi, P Beverley, JW McCauley, RS Daniels, JA Hammond, AR Townsend, E Tchilian
    PloS Pathogens, 2021-03-04;17(3):e1009330.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Porcine CD8alphadim/-NKp46high NK cells are in a highly activated state.
    Authors: Mair K, Mullebner A, Essler S, Duvigneau J, Storset A, Saalmuller A, Gerner W
    Vet Res, 2013-03-01;44(0):13.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. NKp46 expression discriminates porcine NK cells with different functional properties.
    Authors: Mair KH, Essler SE, Patzl M, Storset AK, Saalmuller A, Gerner W
    Eur. J. Immunol., 2012-05-01;42(5):1261-71.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Cell-mediated and humoral immune responses in pigs following primary and challenge-exposure to Lawsonia intracellularis.
    Authors: Cordes H, Riber U, Jensen TK, Jungersen G
    Vet. Res., 2012-02-08;43(1):9.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Activation of Porcine Natural Killer (NK) Cells and Lysis of Foot-and-Mouth Disease Virus (FMDV) Infected Cells.
    Authors: Toka FN, Nfon CK, Dawson H, Mark Estes D, Golde WT
    J. Interferon Cytokine Res., 2009-03-01;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay

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