Recombinant Rat CD19 Fc Chimera Protein, CF

Catalog #: 10525-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10525-CD-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat CD19 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat CD19 Fc Chimera(Catalog # 10525-CD) is immobilized at 5 µg/mL,100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED₅₀ of 10-60 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived rat CD19 protein

Rat CD19 (Arg19-Gly287) Accession # NP_001013255.2	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_001013255.2

N-terminal Sequence
Analysis

Arg19

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

57 kDa

SDS-PAGE

80-95 kDa, under reducing conditions

Product Datasheets

10525-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10525-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD19

CD19, also known as B4, is a transmembrane glycoprotein of the immunoglobulin superfamily that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 consists of an extracellular domain (ECD) with two C2-type Ig-like domains, a transmembrane segment, and a cytoplasmic domain with nine tyrosine residues, 3 of which are critical for function (1,2). Within the mature ECD, rat CD19 shares 57% and 88% amino acid sequence identity with human and mouse CD19, respectively. CD19 is expressed throughout B cell development from pre‑B cells through mature B cells, and it is commonly used as a B cell lineage marker (1, 2). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (1, 2). CD19 associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu-13 (1, 3). These associations enable CD19 to amplify B cell signaling and reduce the threshold for antigen stimulation through the BCR (1, 3). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19+ target cells can be recognized and killed by T cells that express anti-CD19 chimeric antigen receptors (CARs) (4). This interaction has been shown to be beneficial in the treatment of various B-cell malignancies, such as leukemias and lymphomas (4, 5).

References

Wang, K. et al. (2012) Exp. Hematol Oncol. 1:36.
Del Nargo, C.J. et al. (2005) Immunol Res. 31:229.
Yu, F. et al. (2010) J Neurooncol. 103:187.
Kochenderfer, J. et al. (2015) J Clin Oncol. 33:540.
Lee, D. et al. (2015) Lancet. 385:517.

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Alternate Names

B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12