Recombinant Rat CD30/TNFRSF8 Fc Chimera Protein, CF

Recombinant Rat CD30/TNFRSF8 Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CD30/TNFRSF8

Lymphocyte activation antigen CD-30 (CD30), also known as Tumor necrosis factor receptor superfamily member 8 (TNFRSF8), is a type I transmembrane glycoprotein belonging to the TNFR superfamily (1, 2). Mature rat CD30 consists of an extracellular domain (ECD) containing four disulfide bonds and two cysteine-rich repeats, a transmembrane domain, and a cytoplasmic domain. Within the ECD, rat CD30 shares 45% and 79% amino acid (aa) sequence identity with human and mouse CD30, respectively. In human, isoforms of CD30 with truncated cytoplasmic domains can be generated as well as a soluble form, which is produced by proteolytic cleavage of CD30 at the cell surface and is found in serum (3, 4). CD30 is normally expressed on antigen-stimulated Th cells and B cells (5 ‑ 7). CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells (2, 6). Signaling via CD30‑CD30L has been shown to induce the activation and proliferation of T cells and the CD30–CD30L pathway has been implicated as a major player in secondary humoral immune responses (2). In the absence of antigenic stimulation, CD30 can still induce T cell expression of IL‑13 (8). CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells (8, 9). In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5 (6, 10). Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling (11). CD30 is up‑regulated in Hodgkin's disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity (12).

Related Research Areas

• B Cell Development and Differentiation
• Co-stimulatory and Co-inhibitory Molecules
• Co-stimulatory Molecules Associated with Tfh Cells
• Embryonic and Induced Pluripotent Stem Cell Markers
• Lymphoma
• Regulation of Apoptosis by TNF Superfamily Members
• Regulation of B Cell Activation and Homeostasis by TNF Superfamily Members
• Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members
• Regulation of T Cell Co-stimulation by TNF Superfamily Members
• Regulatory T Cells (Tregs)
• TNF Superfamily
• TNF Superfamily Receptors