Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF

Catalog #: 2159-FA
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2159-FA-050

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Citations (3)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED₅₀ for this effect is 0.05-0.25 µg/mL in the presence of 100 ng/mL recombinant rat Fas Ligand.

Source

Mouse myeloma cell line, NS0-derived rat Fas/TNFRSF6/CD95 protein

Rat Fas (Gln22 - Lys170) Accession # NP_631933	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

NP_631933

N-terminal Sequence
Analysis

No results obtained: Gln22 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

43.3 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Product Datasheets

2159-FA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2159-FA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6 (1 ‑ 3). Rat Fas cDNA encodes 324 amino acids (aa) that include a 21 aa signal peptide, a 150 aa extracellular domain (ECD) that contains three cysteine-rich TNFR repeats, a 17 aa transmembrane sequence, and a 136 aa cytoplasmic domain containing a death domain (DD), which is required for transducing apoptotic signals (4). Mature rat Fas ECD shares 68% aa sequence identity with mouse Fas, and 56 ‑ 58% with human, feline, bovine and porcine Fas. A 114 aa potential rat Fas isoform contains an alternate start site at aa 211, thus lacking all three TNFR repeats (5). The Fas ligand (FasL, TNFSF6) is a type II transmembrane protein of the TNF family that can be expressed on activated T-lymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form (2). Engagement of FAS induces oligomerization of preformed Fas trimers (1, 2). The activated receptor recruits the adaptor molecule FADD to form the Death-Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade (6). Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activation-induced death of T lymphocytes that terminates the immune reaction (1, 2, 7). In immune-privileged tissues, infiltrating Fas-bearing lymphocytes and inflammatory cells are killed by FasL engagement (8). Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (1 ‑ 3). The Fas pathway also appears to cross-communicate with the BIM (mitochondrial/intrinsic) apoptosis pathway (1).

References

Bouillet, P. and L.A. O’Reilly (2009) Nat. Rev. Immunol. 9:514.
Strasser, A. et al. (2009) Immunity 30:180.
Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
SwissProt accession Q63199.
GenBank protein accession EDM13159.
Thorburn, A. (2003) Cellular Signaling 16:139.
Barreiro, R. et al. (2004) J. Immunol. 173:1519.
Ferguson, T.A. and T.S Griffith (2006) Immunol. Rev. 213:228.

Long Name

Fibroblast-associated

Entrez Gene IDs

355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)

Alternate Names

Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6

Related Research Areas

Citations for Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Apolipoprotein E is a pancreatic extracellular factor that maintains mature ?-cell gene expression
Authors: AI Mahmoud, FX Galdos, KA Dinan, MP Jedrychows, JC Davis, A Vujic, I Rachmin, C Shigley, JR Pancoast, S Lee, J Hollister-, CM MacGillivr, SP Gygi, DA Melton, GC Weir, RT Lee
PLoS ONE, 2018-10-10;13(10):e0204595.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
c-Jun N-terminal kinases mediate Fas-induced neurite regeneration in PC12 cells.
Authors: Waetzig V, Loose K, Haeusgen W, Herdegen T
Biochem. Pharmacol., 2008-07-19;76(11):1476-84.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Murine coronavirus-induced oligodendrocyte apoptosis is mediated through the activation of the Fas signaling pathway.
Authors: Liu Y, Zhang X
Virology, 2006-12-06;360(2):364-75.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay