Recombinant Rat FGF-10 Protein

Catalog #: 7804-FG Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
7804-FG-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
7804-FG-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat FGF-10 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED₅₀ for this effect is 20-120 ng/mL.

Source

E. coli-derived rat FGF-10 protein
Gln37-Ser215, with an N-terminal Met

Accession #

P70492

N-terminal Sequence
Analysis

Met

Structure / Form

Monomer

Predicted Molecular Mass

20.2 kDa

SDS-PAGE

22 kDa, reducing conditions

Product Datasheets

7804-FG (with carrier)

Product Datasheet

COA

SDS

7804-FG/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7804-FG

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7804-FG/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and DTT.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: FGF-10

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature rat FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2). It shares 96% and 91% amino acid sequence identity with human and mouse FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 3). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (4). FGF-10 plays an important role in many tissues during embryogenesis. This includes limb bud initiation (5), palate development (6), branching morphogenesis and directional outgrowth of lung buds (7, 8), formation of the otic vesicle and chochlea (9), adipogenesis (10), folliculogenesis (11), cardiomyocyte differentiation (12), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (13‑16). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (6, 9, 14‑16). The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung (7, 8). Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF-10 biology (6, 8, 15, 16). FGF-10 signaling through FGF RIIIb also contributes to the progression of pancreatic cancer (17).

References