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Recombinant Rat GITR/TNFRSF18 Fc Chimera Protein, CF

Catalog #: 9130-GR
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9130-GR-050
Recombinant Rat GITR/TNFRSF18 Fc Chimera Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat GITR/TNFRSF18 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat GITR/TNFRSF18 Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Mouse GITR Ligand/TNFSF18 aa 47-173 (Catalog # 2177-GL) that produces 50% of the optimal binding response is 25-150 ng/mL
Source
Mouse myeloma cell line, NS0-derived rat GITR/TNFRSF18 protein
Rat GITR/TNFRSF18
(Glu25-His153)
Accession # NP_001019520		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus
Accession #
NP_001019520
N-terminal Sequence
Analysis
Glu25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41 kDa
SDS-PAGE
55-64 kDa, reducing conditions

Product Datasheets

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9130-GR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9130-GR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Rat GITR/TNFRSF18 Fc Chimera Protein Binding Activity View Larger

When Recombinant Rat GITR/TNFRSF18 Fc Chimera is immobilized at 2 µg/mL,Recombinant Mouse GITR Ligand/TNFSF18 aa 47-173 (Catalog # 2177-GL) binds with an ED50 of 25-150 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature rat GITR consists of a 134 amino acid (aa) extracellular domain (ECD) with three tandem TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain. Within the ECD, rat GITR shares 60% and 86% aa sequence identity with human and mouse GITR, respectively. Alternative splicing generates additional isoforms with an N-terminal extension or a substitution in the cytoplasmic domain. GITR is expressed on CD4+CD25+ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (3-5), and it is up-regulated on antigen-activated conventional CD4+ and CD8+ T cells (4-7). GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4+ or CD8+ conventional T cells (6-9). It also induces the proliferation of Treg (8, 10) but inhibits the ability of Treg to suppress immune responses (3, 8, 11-13). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (3, 11), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (10, 14). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (15).

References
  1. Clouthier, D.L. and T.H. Watts (2014) Cytokine Growth Factor Rev. 25:91.
  2. Ronchetti, S. et al. (2015) J. Immunol. Res. 2015:171520.
  3. Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
  4. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  5. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
  6. Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
  7. Muriglan, S.J. et al. (2004) J. Exp. Med. 200:149.
  8. Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
  9. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
  10. Ephrem, A. et al. (2013) Eur. J. Immunol. 43:2421.
  11. Ko, K. et al. (2005) J. Exp. Med. 202:885.
  12. Ji, H. et al. (2004) J. Immunol. 172:5823.
  13. Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
  14. Patel, M. et al. (2005) Eur. J. Immunol. 35:3581.
  15. O'Keeffe, G.W. et al. (2008) Nat. Neurosci. 11:135.
Long Name
Glucocorticoid Induced TNF Receptor Family Related Gene
Entrez Gene IDs
8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
Alternate Names
Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18

Citation for Recombinant Rat GITR/TNFRSF18 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. GITR/GITRL reverse signalling modulates the proliferation of hepatic progenitor cells by recruiting ANXA2 to phosphorylate ERK1/2 and Akt
    Authors: Y He, Y Pei, K Liu, L Liu, Y Tian, H Li, M Cong, T Liu, H Ma, H You, J Jia, D Zhang, P Wang
    Cell Death & Disease, 2022-04-04;13(4):297.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture

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