Recombinant Rat IFN-beta Protein

Catalog #: 13400-1 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
13400-1

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Rat IFN-beta Protein Summary

Product Specifications

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. As there is no international Rat IFN-beta standard, units are determined with respect to the international reference standard for mouse interferon alpha/beta (mouse IFN-alpha / beta ) provided by the National Institutes of Health [see Pestka, S. (1986) “Interferon Standards and General Abbreviations, ” in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14- 23]. Units of activity measured on rat epithelial-like (RATEC) cells with VSV.

Source

Chinese Hamster Ovary cell line, CHO-derived rat IFN-beta protein

Accession #

D87919

Predicted Molecular Mass

19.68 kDa

Product Datasheets

13400-1

Product Datasheet

COA

SDS

13400-1

Formulation	prior to lyophilization: phosphate buffered saline (PBS); 125mM trehalose
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name

Interferon beta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Alternate Names

Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956