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Recombinant Rat IL-10 Protein

Catalog #: 522-RL
14 Citations 1 Review Datasheet / COA / SDS
Newer Version Available: 522-RLB
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Discontinued Product

522-RL has been discontinued and is replaced by 522-RLB.

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Citations (14)
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat IL-10 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is typically 0.4-2.4 ng/mL.
Source
E. coli-derived rat IL-10 protein
Ser19-Asn178
Accession #
P29456.1
N-terminal Sequence
Analysis
Ser19
Predicted Molecular Mass
19 kDa (monomer)

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522-RL (with carrier)

Product Datasheet
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522-RL/CF (carrier free)

Discontinued Product

522-RL

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL‑19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature rat IL‑10 shares 85% amino acid sequence identity with mouse and 71%-79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8-10). The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12-14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15-17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

References
  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Goodman, R. et al. (1992) Biochem. Biophys. Res. Commun. 189:1.
  9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  10. Syto, R. et al. (1998) Biochemistry 37:16943.
  11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Citations for Recombinant Rat IL-10 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia
    Authors: S Kim, Y Son
    International Journal of Molecular Sciences, 2021-08-16;22(16):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Microglia Responses to Pro-inflammatory Stimuli (LPS, IFN?+TNF?) and Reprogramming by Resolving Cytokines (IL-4, IL-10)
    Authors: S Lively, LC Schlichter
    Front Cell Neurosci, 2018-07-24;12(0):215.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Analytical performance of a commercial multiplex Luminex-based cytokine panel in the rat
    Authors: T Erkens, N Goeminne, A Kegels, M Byloos, P Vinken
    J Pharmacol Toxicol Methods, 2018-01-31;91(0):43-49.
    Species: Rat
    Sample Types: Serum
    Applications: ELISA (Standard)
  4. A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN?
    Authors: JE Lim, E Chung, Y Son
    Sci Rep, 2017-08-25;7(1):9417.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion
    Authors: L Cambier, G de Couto, A Ibrahim, AK Echavez, J Valle, W Liu, M Kreke, RR Smith, L Marbán, E Marbán
    EMBO Mol Med, 2017-03-01;9(3):337-352.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Correlation of Gut Microbiota Composition with Resistance to Experimental Autoimmune Encephalomyelitis in Rats
    Front Microbiol, 2016-12-15;7(0):2005.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  7. Interleukin-10 Contributes to Therapeutic Effect of Mesenchymal Stem Cells for Acute Liver Failure via Signal Transducer and Activator of Transcription 3 Signaling Pathway.
    Authors: Hu-Cheng Ma, Xin Wang, Min-Na Wu, Xin Zhao, Xian-Wen Yuan, Xiao-Lei Shi
    Chinese Medical Journal, 2016-04-20;0(0):0366-6999.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. Expression and contributions of TRPM7 and KCa2.3/SK3 channels to the increased migration and invasion of microglia in anti-inflammatory activation states.
    Authors: Siddiqui T, Lively S, Ferreira R, Wong R, Schlichter L
    PLoS ONE, 2014-08-22;9(8):e106087.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. LIMLE, a new molecule over-expressed following activation, is involved in the stimulatory properties of dendritic cells.
    Authors: Le Texier L, Durand J, Lavault A, Hulin P, Collin O, Le Bras Y, Cuturi M, Chiffoleau E
    PLoS ONE, 2014-04-04;9(4):e93894.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.
    Authors: Steelman A, Smith R, Welsh C, Li J
    J Biol Chem, 2013-07-08;288(33):23776-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
    Authors: Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
    Invest. Ophthalmol. Vis. Sci., 2008-05-16;49(9):3999-4007.
    Species: Rat
    Sample Types: Aqueous Humor
    Applications: Bioassay
  12. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
    Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
    Stem Cells, 2008;26(4):1047-55.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Developmet
  13. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
    Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
    Crit. Care Med., 2005-10-01;33(10):2317-22.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  14. Lipopolysaccharide-induced increase in signalling in hippocampus is abrogated by IL-10--a role for IL-1 beta?
    Authors: Walsh C, Delaney A, Nolan Y, Campbell VA
    J. Neurochem., 2004-02-01;88(3):635-46.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Rat IL-10 Protein
By Anonymous on 11/08/2017
Application: Immunoassay Standard

The recombinant rat IL-10 worked well in building a calibration curve for an ELISA. MAB519 and BAF519 were the antibodies used to build the rat IL-10 ELISA.

Immunoassay Standard Recombinant Rat IL-10 Protein 522-RL