Recombinant Rat IL-13 Protein Summary
Product Specifications
Thr19-His131
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1945-RL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1945-RL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-13
IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1 - 3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature rat IL-13 shares 59%, 75%, and 60% amino acid sequence identity with human, mouse, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11 - 14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).
- Wills-Karp, M. (2004) Immunol. Rev. 202:175.
- Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
- Lakkis, F.G and E.N. Cruet (1993) Biochem. Biophys. Res. Commun. 197:612.
- Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
- Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
- Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
- Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
- Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
- Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
- Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
- Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
- Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387
- Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun.360:464.
- Tabata, Y. et al. (2007) J. Immunol. 177:7905.
- Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
- Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
- Andrews, A.-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
Citations for Recombinant Rat IL-13 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Ethanol Induction of Innate Immune Signals Across BV2 Microglia and SH-SY5Y Neuroblastoma Involves Induction of IL-4 and IL-13
Authors: CJ Lawrimore, LG Coleman, J Zou, FT Crews
Brain Sci, 2019-09-10;9(9):.
Species: Rat
Sample Types: Tissue Explant
Applications: Bioassay -
A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN?
Authors: JE Lim, E Chung, Y Son
Sci Rep, 2017-08-25;7(1):9417.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.
Authors: Steelman A, Smith R, Welsh C, Li J
J Biol Chem, 2013-07-08;288(33):23776-87.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
Cell. Signal., 2008-10-12;21(1):151-60.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
Authors: Miller R, Sadhukhan R, Wu C
J. Immunol. Methods, 2008-03-07;334(1):134-41.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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View all Proteins and Enzyme FAQsReviews for Recombinant Rat IL-13 Protein
Average Rating: 4.5 (Based on 2 Reviews)
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Reason for Rating: Observed good biological activity in vitro.
Reason for Rating: Worked like a charm for stimulating an IL-13Ra1 signaling reporter cell line (293T).
Added IL-13 10ng/mL and 100ng/mL to full culture media overnight and measured secreted reporter the next day.
