Recombinant Rat IL-17A/F Heterodimer Protein Summary
Product Specifications
| Rat IL-17A (Ala18-Ser150 (Ile46Leu)) Accession # Q61453 |
| Rat IL-17F (Arg29-Ala161) Accession # Q5BJ95 | ||
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9340-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
9340-IL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Rat IL‑17A/F Heterodimer (Catalog # 9340-IL) induces IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 3-18 ng/mL.
Reconstitution Calculator
Background: IL-17A/F Heterodimer
Rat IL-17A/F is an approximately 37 kDa, secreted, disulfide-linked glycosylated heterodimeric protein comprised of
two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members
of this family demonstrate a structural motif termed a cysteine knot that also
characterizes a large superfamily of growth factors such as TGF-beta (1).
Although most cysteine knot superfamily members use three intrachain disulfide
bonds to create a knot, IL-17 family molecules generate the same structural
form with only two disulfide links (1, 3-5). Mature rat IL-17A and IL-17F share
60% and 59% amino acid sequence identity with human and 61% and 90% with mouse
IL-17A and IL-17F, respectively. They also share 50% aa sequence identity with
each other (1). IL-17A/F heterodimer and the IL-17A and IL-17F homodimers are
produced by IL-23 activated Th17 cells (1, 6-10, 14). The widely expressed
receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of
IL-17A
and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F
is a biologically active protein that induces chemokine production and airway
neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F
(least potent) (7, 12). It is up-regulated in immune cells during inflammatory
arthritis and contributes to disease severity (13).
- Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
- Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
- Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
- Kolls, J.K. and A. Linden (2004) Immunity 21:467.
- Gu, C. et al. (2013) Cytokine 64:477.
- Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
- Liang, S.C. et al. (2007) J. Immunol. 179:7791.
- Ouyang, W. et al. (2008) Immunity 28:454.
- Steinman, L. (2007) Nat. Med. 13:139.
- Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
- Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
- Wright, J.F. et al. (2008) J. Immunol. 181:2799.
- Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.
- Spolski, R. et al. (2009) Eur. J. Immunol. 3: 658.
Citation for Recombinant Rat IL-17A/F Heterodimer Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Chronic infusion of interleukin-17 promotes hypertension, activation of cytolytic natural killer cells, and vascular dysfunction in pregnant rats
Authors: OK Travis, D White, WA Pierce, Y Ge, CY Stubbs, FT Spradley, JM Williams, DC Cornelius
Physiol Rep, 2019-04-01;7(7):e14038.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
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