Recombinant Rat Nogo-A Fc Chimera (aa 1-172) Protein, CF Summary
Product Specifications
| Rat Nogo-A (Met1-Val172) Accession # Q9JK11 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3098-NG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 400 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Nogo-A
Nogo, so named as a “No-Go” for neurite outgrowth, is a member of the reticulon family of transmembrane proteins, and is also called reticulon 4 (gene name RTN4) (1-4). Reticulons lack N-terminal signal sequences, share a conserved ~200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER‑retention motif, and show a punctate intracellular distribution within the endoplasmic reticulum (ER) that is reminescent of a reticulum (1-3). The N-terminus of intracellular (ER) Nogo-A appears to face the cytoplasm (1-3). However, minor amounts of Nogo-A and Nogo-B are found in the plasma membrane with extracellular N-termini (4-6). Full length rat Nogo-A is a 1163 aa protein with a long (~989 aa) N-terminus that includes bioactive regions (aa 59-172 and 544-725), a transmembrane segment, a connecting loop that contains the bioactive Nogo-66 region, a second transmembrane segment, and a short C-terminus (3). The four Nogo isoforms share the Nogo66 segment, Nogo-A and Nogo-B share aa 1-172, and only Nogo-A contains aa 544-725 (1-3). Rat Nogo-A shares 78% and 91% aa sequence identity with human and mouse Nogo-A, respectively. Rat and human Nogo-A/B also share 78% aa sequence identity within aa 1-172 and 98% within the Nogo-66 loop region. Nogo-A is mainly expressed in oligodendrocytes of the central nervous system, but is also reported in fibroblasts, dorsal root ganglion neurons, macrophages and myoblasts (1-8). Nogo-B is mainly expressed in vascular endothelium and smooth muscle throughout the body (1, 4, 6, 9). The Nogo66 region binds the GPI-linked Nogo receptor/p75 complex on axons, inducing growth cone collapse (5, 7, 10, 11). Either aa 59-172 or 544-725 segments can block neurite outgrowth and fibroblast spreading (5, 6). Nogo-A/B aa 1-172 is also reported to regulate vascular remodeling through binding the Nogo-B receptor (NgBR/NUS1) on vascular cells, and to inhibit neuronal differentiation and promote glial formation from neural progenitors (4, 5, 8, 9, 12).
- Oertle, T. and M.E. Schwab (2003) Trends Cell Biol. 13:187.
- GrandPre, T. et al. (2000) Nature 403:439.
- Chen, M.S. et al. (2000) Nature 403:434.
- Acevedo, L. et al. (2004) Nat. Med. 10:382.
- Oertle, T. et al. (2003) J. Neurosci. 23:5393.
- Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
- Wang, X. et al. (2002) J. Neurosci. 22:5505.
- Schwab, M.E. (2010) Nat. Rev. Neurosci. 11:799.
- Miao, R.Q. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10997.
- Fournier, A.E. et al. (2001) Nature 409:341.
- Wang, K.C. et al. (2002) Nature 420:74.
- Guo, Y. et al. (2009) Neurosci. Lett. 458:132.
Citation for Recombinant Rat Nogo-A Fc Chimera (aa 1-172) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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HspB1 silences translation of PDZ-RhoGEF by enhancing miR-20a and miR-128 expression to promote neurite extension.
Authors: Sun X, Zhou Z, Fink D, Mata M
Mol Cell Neurosci, 2013-10-16;57(0):111-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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