Recombinant Rhesus Macaque IL-13 Protein

Catalog #: 2674-RM
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
2674-RM-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
2674-RM-025

R&D Systems Recombinant Proteins and Enzymes

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Product Details

Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rhesus Macaque IL-13 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 2-8 ng/mL.

Source

E. coli-derived rhesus macaque IL-13 protein
Ser19-Asn132

Accession #

Q864V6.1

N-terminal Sequence
Analysis

Ser19

Predicted Molecular Mass

12.6 kDa

Product Datasheets

2674-RM (with carrier)

Product Datasheet

COA

SDS

2674-RM/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2674-RM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2674-RM/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1, 2). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (3, 4). Mature rhesus IL-13 shares 94%, 58%, and 60% amino acid sequence identity with human, mouse, and rat IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (5, 6). IL-13 has diverse activities on numerous cell types (7). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (8, 9). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (10 - 13). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (9, 14, 15). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (16).

References

Wills-Karp, M. (2004) Immunol. Rev. 202:175.
Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
Tabata, Y. et al. (2007) J. Immunol. 177:7905.
Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
Andrews, A.-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

Long Name

Interleukin 13

Entrez Gene IDs

3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)

Alternate Names

ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600

Related Research Areas

Citation for Recombinant Rhesus Macaque IL-13 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1