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Recombinant SARS-CoV-2 GST-Papain-like Protease, CF

Catalog #: E-611
2 Citations Datasheet / COA / SDS
E. coli Expressed, Bioactivity Tested
Catalog # Availability Size / Price Qty
E-611-050
Recombinant SARS-CoV-2 GST-Papain-like Protease SDS-PAGE
1 Image
Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant SARS-CoV-2 GST-Papain-like Protease, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant SARS-CoV-2 virus Papain-like protease (PLPro) is a Ubiquitin- and ISG15-deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rhodamine 110 (U-555) substrate.  When using polyubiquitin chain substrate(s), this enzyme demonstrates a preference for K48 linkages.
Source
E. coli-derived sars-cov-2 Papain-like Protease protein
Glu746 - Lys1060, with an N-terminal GST-tag
Accession #
YP_009725299.1
Predicted Molecular Mass
62 kDa

Product Datasheets

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E-611

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-611

Formulation Supplied as a solution in HEPES, NaCl, Glycerol and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Scientific Data

SDS-PAGE Recombinant SARS-CoV-2 GST-Papain-like Protease SDS-PAGE View Larger

0.5 μg and 1 μg of Recombinant SARS-CoV-2 GST-Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Papain-like Protease

The Papain-like protease (PLPro) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2 is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  In other coronaviruses, PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminal end of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISG15ylating activity.  In vivo, this protease antagonizes innate immunity by acting on IFN beta and NF- kappa B signaling pathways.  When used in vitro with polyubiquitin substrates, the enzyme demonstrates a strong preference for K48 linkages.  

References
  1. Clasman J.R., et al. (2020) Antiviral Res.  174: 104661.
  2. Frieman M., et al. (2009) J. Virol.  83: 6689.
  3. Lindner H.A., et al. (2007) Arch. Biochem. Biophys.  466: 8.
  4. Ratia K., et al. (2014) PLoS Pathog.  doi: 10.1371/journal.ppat.1004113.
Alternate Names
Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a

Citations for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SARS-CoV-2 papain-like protease activates nociceptors to drive sneeze and pain
    Authors: Mali, SS;Silva, R;Gong, Z;Cronce, M;Vo, U;Vuong, C;Moayedi, Y;Cox, JS;Bautista, DM;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Identification of ebselen and its analogues as potent covalent inhibitors of papain-like protease from SARS-CoV-2
    Authors: E Weglarz-To, JM Tomczak, M Talma, M Burda-Grab, M Giurg, S Brul
    Scientific Reports, 2021-02-11;11(1):3640.
    Species: N/A
    Sample Types: Enzyme
    Applications: Inhibition

FAQs

  1. Is Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) a suitable substrate for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611)?

    • Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611) only cleaves at the C-terminus of ISG15 and/or Ubiquitin conjugates. Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) is not a suitable substrate because the fluorescein-labeled lysine residues are distributed throughout the protein, but not at the C-terminus. Suitable substrate candidates for Catalog #E-611 include include Recombinant Human Ubiquitin AMC Protein, CF (Catalog #U-550), Recombinant Human Ubiquitin Rhodamine 110 Protein, CF (Catalog # U-555), and Recombinant Human ISG15 AMC Protein, CF (Catalog # UL-553).

  2. Is there a molar extinction coefficient for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611)?

    • We have not determined the molar extinction coefficient of E-611 as this is not a regular QC specification. However, a theoretical E280 extinction coefficient can be produced using Expasy software based on the amino acid sequence, which includes the GST tag in the N-terminus.

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