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Recombinant SARS-CoV 3CL Protease, CF

Catalog #: E-718
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E-718-050
Recombinant SARS-CoV 3CL Protease Inhibition Activity
2 Images
Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant SARS-CoV 3CL Protease, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Reaction conditions will need to be optimized for each specific application.
Source
E. coli-derived sars-cov 3CL Protease protein
Ser1 - Gln306 with a C-terminal 6-His tag
Accession #
NP_828863.1
Predicted Molecular Mass
34 kDa

Product Datasheets

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E-718

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-718

Formulation Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Scientific Data

Inhibition Activity Recombinant SARS-CoV 3CL Protease Inhibition Activity View Larger

GC376 achieved 50% inhibition of 50 nM SARS-CoV Virus 3CL Protease (Catalog # E-718) at a concentration of 101-120 nM.

SDS-PAGE View Larger

1 μg and 2 μg of Recombinant SARS-CoV Virus 3CL Protease (Catalog # E-718) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 33 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 3CL Protease

The 3CL protease (aka 3CLpro, Mpro or "Main Protease) from the human SARS-CoV coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide.  3CLpro activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid.  Along with the CoV Papain-Like Protease (catalog number E-610), 3CLpro presents an attractive target for therapeutic intervention of coronavirus infections.  Because no human proteases with a similar cleavage specificity are known, inhibitors of 3CLpro are unlikely to cause mechanism-based toxicity.  

References
  1. Chen, Y.W., et al. (2020) F1000Research 9:129.
  2. Pillaiyar, T., et al. (2016) J. Med. Chem. 59:6595.
Long Name
3CL Protease
Alternate Names
3CL Protease; 3CL Proteinase; 3C-like main protease; 3CL-Mpro; COVID-19; M Proteinase; Main Protease; Mpro

Citations for Recombinant SARS-CoV 3CL Protease, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Novel dithiocarbamates selectively inhibit 3CL protease of SARS-CoV-2 and other coronaviruses
    Authors: L Brier, H Hassan, X Hanoulle, V Landry, D Moschidi, L Desmarets, Y Rouillé, J Dumont, A Herledan, S Warenghem, C Piveteau, P Carré, S Ikherbane, FX Cantrelle, E Dupré, J Dubuisson, S Belouzard, F Leroux, B Deprez, J Charton
    European Journal of Medicinal Chemistry, 2023-02-06;250(0):115186.
    Species: Human
    Sample Types: Reference Standard
  2. All-Trans Retinoic Acid Exhibits Antiviral Effect against SARS-CoV-2 by Inhibiting 3CLpro Activity
    Authors: T Morita, K Miyakawa, SS Jeremiah, Y Yamaoka, M Sada, T Kuniyoshi, J Yang, H Kimura, A Ryo
    Viruses, 2021-08-23;13(8):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

FAQs

  1. Does the Recombinant SARS-CoV 3 CL Protease, CF (Catalog # E-718) contain any linkers or tags in the sequence?

    • Recombinant SARS-CoV 3 CL Protease, CF (Catalog # E-718) is an E. coli derived protein with amino acids Ser3241-Gln3546 that does not contain any linkers or tags in the sequence.

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