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Recombinant T. castaneum His6-MBP-PINK1 Protein, CF

Catalog #: AP-180
3 Citations Datasheet / COA / SDS
 

Discontinued Product

AP-180 has been discontinued.
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Citations (3)
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Recombinant T. castaneum His6-MBP-PINK1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial PINK1 concentration of 0.5-2 µM for the phosphorylation of recombinant Parkin, Ubiquitin, or Polyubiquitin chains.

Source
E. coli-derived t. castaneum PINK1 protein
Contains an N-terminal MBP and 6-His tag
Accession #
 XP_968367.1
Predicted Molecular Mass
108 kDa

Product Datasheets

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AP-180

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-180

Formulation X mg/ml (X μM) in 20 mM HEPES pH 7.6, 50 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PINK1

Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress. PINK is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function. In damaged (depolarized) mitochondria PINK becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Mfn2. Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by 1) PINK-mediated phosphorylation of PARK2 at serine 65, and 2) PARK2 interaction with phosphorylated Ubiquitin (also phosphorylated by PINK on serine 65). This signaling cascade is critical for clearing the damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2.

Recombinant human PINK1 is not active in vitro, while this protein from the Red Flour Beetle (Tribolium castaneum) effectively phosphorylates recombinant Parkin, mono-Ubiquitin, and poly-Ubiquitin chains. It specifically phosphorylates both Parkin and Ubiquitin at serine 65. This recombinant protein contains N-terminal 6-His and MBP tags.

References
  1. Kane L.A., et al. (2014) J. Cell Biol. 205: 143
  2. Matsuda N., et al. (2010) J. Cell Biol. 189: 211
  3. Vives-Bauza C., et al. (2010) Proc. Natl. Acad. Sci. 107: 378
  4. Wauer T., et al. (2015) EMBO J. 34: 307
Long Name
PTEN-induced Putative Kinase 1
Entrez Gene IDs
65018 (Human); 68943 (Mouse); 298575 (Rat); 3925520 (T. castaneum)
Alternate Names
BRPK; EC 2.7.11.1; FLJ27236; PARK6; Parkinson disease (autosomal recessive) 6; PINK1; protein kinase BRPK; PTEN Induced Kinase 1; PTEN induced putative kinase 1; PTEN-induced putative kinase protein 1; serine/threonine-protein kinase PINK1, mitochondrial

Citations for Recombinant T. castaneum His6-MBP-PINK1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease
    Authors: SK Pirooznia, H Wang, N Panicker, M Kumar, S Neifert, MA Dar, E Lau, BG Kang, J Redding-Oc, JC Troncoso, VL Dawson, TM Dawson
    Science Advances, 2022-04-01;8(13):eabh1824.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Parkin is an E3 ligase for the ubiquitin-like modifier FAT10, which inhibits Parkin activation and mitophagy
    Authors: ND Roverato, C Sailer, N Catone, A Aichem, F Stengel, M Groettrup
    Cell Reports, 2021-03-16;34(11):108857.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death
    Authors: H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao
    Cell Death Differ., 2017-12-12;0(0):.
    Applications: Bioassay

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