Recombinant Viral CCI Fc Chimera Protein, CF Summary
Product Specifications
| Viral CCI (Met17-Val258)& (Pro18-Val258) Accession # P19063 | IEGRMD | Human IgG1 (Pro100-Lys330) | 6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
696-CC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Viral CCI Fc Chimera (Catalog # 696-CC) inhibits Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE) induced chemotaxis of BaF3 mouse pro-B cells transfected with human CCR2A. The ED50 for this effect is 0.1-0.5 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL2/JE/MCP-1.
Reconstitution Calculator
Background: CCI
The family of T1/35 kDa proteins are encoded by the '35K' virulence gene of many poxviruses and are secreted from virus-infected cells. These proteins bind
CC-chemokines with high affinity and are termed viral chemokine inhibitor (vCCI). Viral CCI from various poxviruses share multiple stretches of sequence identity and eight conserved cysteine residues (1). The vaccinia virus (strain Lister) vCCI cDNA encodes a 258 amino acid (aa) protein with a putative 17 aa signal peptide (2). Vaccinia virus (strain Lister) vCCI shows greater than 90% aa sequence identity with vCCI from other orthopoxviruses and approximately 40% aa sequence identity with the leporipoxvirus T-1 proteins. vCCI binds with high affinity to many human, mouse, and rat CC-chemokines (1, 3-5). It binds to the same surfaces of these chemokines that are involved in chemokine receptor interactions (4-7). Some CC-chemokines utilize common binding surfaces on vCCI, while others interact with distinct sites on vCCI (8). vCCI inhibits CC-chemokine induced monocyte chemotaxis in vitro but does not block cellular effects induced by CXCL5/ENA-78, CXCL8/IL-8, or CXCL10/IP-10 (3-5). In vivo, vCCI restricts the infiltration of immune cells into sites of inflammation (3, 9, 10) and also the CC-chemokine induced migration of arterial vascular smooth muscle cells (11).
- Graham, K.A. et al. (1997) Virology 229:12.
- Patel, A.H. et al. (1990) J. Gen. Virol. 71:2013.
- Buatois, V. et al. (2010) J. Immunol. 185:2544.
- Smith, C.A. et al. (1997) Virology 236:316.
- Lalani, A.S. et al. (1998) Virology 250:173.
- Beck, C.G. et al. (2001) J. Biol. Chem. 276:43270.
- Seet, B.T. et al. (2001) Proc. Natl. Acad. Sci. 98:9008.
- Burns, J.M. et al. (2002) J. Biol. Chem. 277:2785.
- Dabbagh, K. et al. (2000) J. Immunol. 165:3418.
- Reading, P.C. et al. (2003) J. Immunol. 170:1435.
- Spinetti, G. et al. (2004) Arterioscler. Throm. Vasc. Biol. 24:1397.
Citation for Recombinant Viral CCI Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis.
Authors: Buatois V, Fagete S, Magistrelli G, Chatel L, Fischer N, Kosco-Vilbois MH, Ferlin WG
J. Immunol., 2010-07-19;185(4):2544-54.
Species: Human
Sample Types: Serum
Applications: ELISA (Capture)
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