Does viral IL-10 cross-react in bioassay?

Name: Recombinant Viral EBV IL-10 Protein
Brand: R&D Systems
SKU: 915-VL
Price: 447 USD

In an MC9 bioassay, recombinant EBV IL-10 is 20 times less active than recombinant human IL-10.

Recombinant Viral EBV IL-10 Protein

Catalog #: 915-VL
3 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
915-VL-010/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
915-VL-010

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Product Details

Citations (3)

FAQs

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Viral EBV IL-10 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED₅₀ for this effect is 3‑15 ng/mL.

Source

E. coli-derived viral IL-10 protein
Gln26-Arg170, with an N-terminal Met

Accession #

P03180

N-terminal Sequence
Analysis

Met

Structure / Form

Noncovalently-linked homodimer

Predicted Molecular Mass

17.2 kDa

Product Datasheets

915-VL (with carrier)

Product Datasheet

COA

SDS

915-VL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

915-VL

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.

915-VL/CF

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: IL-10

Interleukin 10 (IL-10) is a pleiotropic cytokine that plays an important role in regulating inflammatory and immune responses. It is a potent suppressant of proinflammatory cytokine production by monocytes/macrophages and neutrophils. It is also an inhibitor of macrophage and T cell effector functions. Homologs of mammalian IL-10 have been identified in the genome of several viruses, including Epstein-Barr virus (EBV), poxvirus Orf, baboon cytomegalovirus, and human and equine herpes virus. In the EBV genome, the BCRF1 open reading frame encodes the EBV IL-10 (vIL-10). The viral IL-10 precursor is a 170 amino acid residue (aa) protein with a putative 25 aa signal peptide that is cleaved to yield the 145 aa mature protein. The EBV IL-10 precursor shares approximately 78% and 65% amino acid sequence homology with human and mouse IL-10, respectively. Most of the deviations are localized to the signal peptide and the first 20 amino-terminal residues. Viral IL-10 is expressed during the late phase of the lytic cycle of EBV infection. Viral IL-10 is a partial agonist of mammalian IL-10 and shares many of their activities. It is likely that vIL-10 may have a role in host immune evasion.

References

Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Related Research Areas

Citations for Recombinant Viral EBV IL-10 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia
Authors: J Stuchl?, V Kanderov , M Vlkov , I He?manov , L Sl mov , O Pel k, E Taraldsrud, D J¡lek, P Kr l¡c Kov, B Fevang, M Trkov , O Hru? k, E Fro?kov , A ?ediv , J Litzman, T Kalina
Sci Rep, 2017-01-05;7(0):39710.
Applications: ELISA (Standard)
Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
Authors: Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C
BMC Biotechnol, 2013-10-05;13(0):82.
Species: Mouse
Sample Types: N/A, Whole Cells
Applications: Bioassay, Western Blot
Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
Authors: Chang WL, Baumgarth N, Yu D, Barry PA
J. Virol., 2004-08-01;78(16):8720-31.
Species: Virus
Sample Types: Whole Cells
Applications: Bioassay