Recombinant Viral JEV-E Fc Chimera Protein, CF
Discontinued Product
Recombinant Viral JEV-E Fc Chimera Protein, CF Summary
Product Specifications
| Viral Japanese Encephalitis virus Envelope (Phe295-Leu746) Accession # P32886 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9449-JV
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.
Reconstitution Calculator
Background: Japanese Encephalitis virus Envelope
JEV_E is the envelope protein E produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the sole agent of Japanese encephalitis (1). Members of this family of viral pathogens have a single, positive-strand RNA genome approximately 11 kb that encodes seven non-structural, and three structural proteins: capsid (C), precursor to membrane (prM) and envelope (E). The JEV_E protein consists of 500 aa that include two transmembrane helices, and there is ~40% aa identity among the flavivirus E proteins (2). The E protein is an approximately 53 kDa elongated protein that contains cellular receptor-binding sites and fusion peptide and is mainly responsible for interaction with the host cell, infectivity, and entry of the virus (2-6).
- Banerjee, S. et al. (2017) BMC Immunol. 18:13.
- Mukhopadhyay, S. et al. (2005) Nat. Rev. Microbiol. 3:13.
- Lorenz, I.C. et al. (2002) J. Virol. 76:5480.
- Allison, S.L. et al. (2001) J. Virol. 75:4268.
- Liu, H. et al. (2015) J. Virol. 89:5668.
- Yang, J. et al. (2017) Viruses 9:20.
FAQs
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