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Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF

Catalog #: E-300
6 Citations Datasheet / COA / SDS

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Will be discontinued when existing inventory is gone
Catalog # Availability Size / Price Qty
E-300-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Recombinant Yeast GST-Ubiquitin E1 Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.   We recommend an initial GST-Ubiquitin E1 Enzyme (UBE1) concentration of 50-200 nM.

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived yeast Ubiquitin-activating Enzyme/UBE1 protein
Met1 - Leu1024 with a N-terminal GST (glutathione S-transferase) tag
Accession #
P22515.2
Predicted Molecular Mass
141 kDa

Product Datasheets

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E-300

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-300

Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin-activating Enzyme/UBE1

Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1024 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 114 kDa.  Human and mouse UBE1 share 52% and 53% aa sequence identity with the yeast UBE1 protein, respectively.  UBE1 is found in the cytoplasm and nucleus, and contains a conserved active site cysteine residue and ATP-binding site common to E1 enzymes (1-3).  UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5).  Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys600 in S. cerevisiae, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8).  The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating-Ubiquitin ligase enzyme cascade.  UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11).  UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.  

References
  1. Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
  2. Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
  3. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
  4. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  5. Schulman, B.A. and J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
  6. Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
  7. Haas, A.L. and I.A. Rose (1982) J. Biol. Chem. 257:10329.
  8. Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
  9. Nouspikel, T. and P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
  10. Moundry, P. et al. (2012) Cell Cycle 11:1573.
  11. Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.
Entrez Gene IDs
7317 (Human); 22201 (Mouse); 314432 (Rat); 100009225 (Rabbit); 853670 (Yeast)
Alternate Names
A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; GXP1; MGC4781; POC20 centriolar protein homolog; POC20; Protein A1S9; SMAX2; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1A1S9; UBE1X; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing); Ubiquitin-activating enzyme E1; Ubiquitinactivating Enzyme; Ubiquitin-activating Enzyme; ubiquitin-like modifier activating enzyme 1; ubiquitin-like modifier-activating enzyme 1

Citations for Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. PTPN23 ubiquitination by WDR4 suppresses EGFR and c-MET degradation to define a lung cancer therapeutic target
    Authors: Singh, S;Yeat, NY;Wang, YT;Lin, SY;Kuo, IY;Wu, KP;Wang, WJ;Wang, WC;Su, WC;Wang, YC;Chen, RH;
    Cell death & disease
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Structure-function analysis of histone H2B and PCNA ubiquitination dynamics using deubiquitinase-deficient strains
    Authors: Radmall, KS;Shukla, PK;Leng, AM;Chandrasekharan, MB;
    Scientific reports
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. SCFRMF mediates degradation of the meiosis-specific recombinase DMC1
    Authors: Xu, W;Yu, Y;Jing, J;Wu, Z;Zhang, X;You, C;Ma, H;Copenhaver, GP;He, Y;Wang, Y;
    Nature communications
    Species: Plant
    Sample Types: Cell Lysates
    Applications: Bioassay
  4. Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex
    Authors: PK Shukla, JE Bissell, S Kumar, S Pokhrel, S Palani, KS Radmall, O Obidi, TJ Parnell, J Brasch, DC Shrieve, MB Chandrasek
    Nucleic Acids Research, 2023-03-21;0(0):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126 affect ubiquitination activity and decrease enzyme stability
    Authors: PK Shukla, D Sinha, AM Leng, JE Bissell, S Thatipamul, R Ganguly, KS Radmall, JJ Skalicky, DC Shrieve, MB Chandrasek
    The Journal of Biological Chemistry, 2022-09-23;0(0):102524.
    Species: Yeast
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Ubiquitin turnover and endocytic trafficking in yeast are regulated by Ser57 phosphorylation of ubiquitin
    Authors: S Lee, JM Tumolo, AC Ehlinger, KK Jernigan, SJ Qualls-His, PC Hsu, WH McDonald, WJ Chazin, JA MacGurn
    Elife, 2017-11-13;6(0):.
    Species: Yeast
    Sample Types: Protein
    Applications: Bioassay

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