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SARS CoV-2 3CL Protease Substrate, Rh110-conjugated

Catalog #: S-720 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
S-720-200
Structure of LGSAVLQ-Rh110-dP
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

SARS CoV-2 3CL Protease Substrate, Rh110-conjugated Summary

Product Specifications

Purity
>95%, by HPLC.
Activity
LGSAVLQ-Rh110-dP is a fluorogenic substrate for measuring the 3CL protease activity of coronaviruses.  The sequence is derived from the SARS CoV-2 3CLpro.  Release of Rhodamine 110 can be monitored with excitation and emission wavelengths of 485 nm and 535 nm, respectively.  Conditions will need to be optimized for each specific application.  We recommend an initial substrate concentration of 4-20 μM.
Source
Chemically Synthesized
LGSAVLQ-rhodamine 110-dP
Predicted Molecular Mass
1096.3 Da

Product Datasheets

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S-720

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

S-720

Formulation 4 mM in DMSO
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 3CL Protease

The 3CL protease (aka 3CLpro, Mpro or "Main" Protease) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide.  3CLpro activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid.  Along with the CoV-2 Papain-Like Protease (catalog number E-611), 3CLpro presents an attractive target for therapeutic intervention for COVID-19.  Because no human proteases with a similar cleavage specificity are known, inhibitors of 3CLpro are unlikely to cause mechanism-based toxicity.


This peptide substrate (LGSAVLQ-Rh110-dP) contains a naturally occurring cleavage site for SARS-CoV-2 3CL protease conjugated to a Rhodamine 110 dye.  3CL protease activity releases the dye to fluoresce with high signal-to-background ratios in either kinetic or end-point assays.

References
  1. Chen Y.W., et al. (2020) F1000Research  doi: 10.12688/f1000research.22457.2
  2. Pillaiyar T., et al. (2016) J. Med. Chem.  59: 6595
  3. Zhang L., et al. (2020) Science  doi: 10.1126/science.abb3405
Long Name
3CL Protease
Alternate Names
3CL Protease; 3CL Proteinase; 3C-like main protease; 3CL-Mpro; COVID-19; M Proteinase; Main Protease; Mpro

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