sCy5DL-amide

Catalog # Availability Size / Price Qty
7835/2
sCy5DL-amide | Fluorescent Probes for Imaging Bacteria
2 Images
Description: FDAA for super-resolution microscopy of bacteria

Chemical Name: 2-((1E,3E)-5-((E)-1-(6-(((R)-6-Amino-5-ammonio-6-oxohexyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)penta-1,3-dien-1-yl)-1,3,3-trimethyl-3H-indol-1-ium-5-sulfonate

Purity: ≥95%

Product Details
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Biological Activity

sCy5DL-amide is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into Gram-positive B.subtilis. sCy5DL-amide labeled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/666 nm.

Scientific Data

Fluorescence Imaging Bacillus subtilis cells stained with sCy5DA-amide View Larger

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA-amide

Optical Data for sCy5DL-amide

sCy5DL-amide Dye Spectra
Emission Color Red
λabs 646 nm
λem 666 nm
Extinction Coefficient (ε) 209,200 M-1cm-1
Cell Permeable Yes
Application STORM

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
769.97
Formula:
C38H51N5O8S2
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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