Simple Plex Cell Activation Panel 1
Simple Plex Cell Activation Panel 1 Summary
The Simple Plex Human Multianalyte Assay is for the detection of human GRANB, IFN gamma, IL-2, and TNF-alpha in Cell Culture Supernates.| Sample Type & Volume Required Per Well | Cell Culture Supernates (25 uL) |
| Sensitivity | GRANB 1.31 pg/mL; IFN gamma 0.17 pg/mL; IL-2 0.54 pg/mL; TNF-alpha 0.3 pg/mL |
| Assay Range | GRANB 1.31-5,000 pg/mL; IFN gamma 0.17-4,000 pg/mL; Il-2 0.54-2,050 pg/mL; TNF-alpha 0.3-1,160 pg/mL |
Product Specifications
| Reactivity | Human |
| Specificity | Natural and recombinant human GRANB, IFN gamma, IL-2 and TNF alpha. |
| Cross-reactivity | <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
| Intra-Assay Precision | Inter-Assay Precision | ||||
|---|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 | |
| GRANB | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 28 | 1357 | 28.7 | 1377 | |
| Standard Deviation | 1.26 | 47.1 | 2.87 | 143 | |
| CV (%) | 4.5 | 3.5 | 10 | 10.4 | |
| IFN gamma 3rd gen | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 21.1 | 1175 | 23.4 | 1192 | |
| Standard Deviation | 1.48 | 33.4 | 2.52 | 99.4 | |
| CV (%) | 7 | 2.8 | 10.8 | 8.3 | |
| IL-2 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 15 | 648 | 13 | 608 | |
| Standard Deviation | 0.8 | 32.7 | 1.13 | 34 | |
| CV (%) | 5.3 | 5.1 | 8.7 | 5.6 | |
| TNF alpha 2nd Gen | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 6.99 | 383 | 0.662 | 36.9 | |
| Standard Deviation | 0.345 | 20.6 | 7.81 | 408 | |
| CV (%) | 4.9 | 5.4 | 8.5 | 9 | |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % | |
|---|---|---|---|
| GRANB | EDTA Plasma (n=4) | 103 | 91-117 |
| Heparin Plasma (n=4) | 95 | 86-107 | |
| Serum (n=4) | 105 | 91-119 | |
| IFN gamma | EDTA Plasma (n=4) | 89 | 88-90 |
| Heparin Plasma (n=4) | 90 | 83-99 | |
| Serum (n=4) | 95 | 83-102 | |
| IL-2 | EDTA Plasma (n=4) | 86 | 69-1002 |
| Heparin Plasma (n=4) | 90 | 70-103 | |
| Serum (n=4) | 92 | 69-102 | |
| TNF alpha 2nd Gen | EDTA Plasma (n=4) | 97 | 89-105 |
| Heparin Plasma (n=4) | 89 | 77-96 | |
| Serum (n=4) | 99 | 92-108 |
Specifications
Product Datasheets
FAQs
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