Simple Plex Cytokine Screening Panel
Simple Plex 32x8 panel containing IL-1b, IL-12 p70, IL-10, IL-4 2nd gen, IL-2, IFNg 3rd gen, TNF-a 2nd gen, and IL-6 2nd Gen with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum)
Simple Plex Cytokine Screening Panel Summary
The Simple Plex Human Multianalyte Assay is for the detection of human IL-1b, IL-12 p70, IL-10, IL-2, IFNg 3rd gen, TNF-a 2nd gen, IL-4 2nd gen, and IL-6 2nd gen in serum and plasma (EDTA/Heparin).| Sample Type & Volume Required Per Well | Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL) |
| Sensitivity | IL-1 beta 0.4 pg/mL; IL-12 p70 0.46 pg/mL; IL-10 0.58 pg/mL; IL-2 0.54 pg/mL; IFNg 3rd gen 0.17 pg/mL; TNF-a 0.3 pg/mL; IL-4 0.52 pg/mL; IL-6 0.28 pg/mL |
| Assay Range | IL-1 beta 0.4 - 1,530 pg/mL; IL-12 p70 0.46 - 2,570 pg/mL; IL-10 0.58 - 2,212 pg/mL; IL-2 0.54 - 2,050 pg/mL; IFNg 3rd gen 0.17 - 4,000 pg/mL; TNF-a 0.3 - 1,160 pg/mL; IL-4 0.52 - 2,020 pg/mL; IL-6 0.28 - 2,652 pg/mL |
Product Specifications
| Reactivity | Human |
| Specificity | Natural and recombinant human IL-1b, IL-12 p70, IL-10, IL-2, IFNg 3rd gen, TNF-a 2nd gen, IL-4 2nd gen, and IL-6 2nd gen. |
| Cross-reactivity | <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
| Intra-Assay Precision | Inter-Assay Precision | ||||
|---|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 | |
| IL-1 beta/IL-1F2 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 8.66 | 446 | 8.42 | 410 | |
| Standard Deviation | 0.427 | 16.5 | 0.342 | 23.3 | |
| CV (%) | 4.9 | 3.7 | 4 | 5.7 | |
| IL-12 p70 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 36.2 | 1657 | 33.2 | 1587 | |
| Standard Deviation | 1.25 | 90 | 1.95 | 137 | |
| CV (%) | 3.4 | 5.4 | 5.9 | 8.6 | |
| IL-10 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 33 | 1591 | 33.2 | 1681 | |
| Standard Deviation | 1.55 | 95.1 | 2.43 | 120 | |
| CV (%) | 4.6 | 6 | 7.1 | 7.1 | |
| IL-2 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 15 | 648 | 13 | 608 | |
| Standard Deviation | 0.8 | 32.7 | 1.13 | 34 | |
| CV (%) | 5.3 | 5.1 | 8.7 | 5.6 | |
| IFN-g | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 21.1 | 1175 | 23.4 | 1192 | |
| Standard Deviation | 1.48 | 33.4 | 2.52 | 99.4 | |
| CV (%) | 7 | 2.8 | 10.8 | 8.3 | |
| TNF-a | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 6.99 | 383 | 0.662 | 36.9 | |
| Standard Deviation | 0.345 | 20.6 | 7.81 | 408 | |
| CV (%) | 4.9 | 5.4 | 8.5 | 9 | |
| IL-4 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 15.9 | 621 | 13.5 | 631 | |
| Standard Deviation | 1.2 | 60.8 | 1.55 | 77.1 | |
| CV (%) | 7.5 | 9.8 | 11.5 | 12.2 | |
| IL-6 | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 37.9 | 1763 | 38.7 | 1709 | |
| Standard Deviation | 0.804 | 108 | 3.95 | 163 | |
| CV (%) | 2.1 | 6.1 | 10.2 | 9.6 | |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % | |
|---|---|---|---|
| IL-1 beta | EDTA Plasma (n=4) | 106 | 97-120 |
| Heparin Plasma (n=4) | 99 | 99-112 | |
| Serum (n=4) | 105 | 96-112 | |
| IL-12 p70 | EDTA Plasma (n=6) | 87 | 73-117 |
| Serum (n=6) | 81 | 73-90 | |
| IL-10 | EDTA Plasma (n=6) | 105 | 88-113 |
| Heparin Plasma (n=6) | 102 | 89-118 | |
| Serum (n=6) | 94 | 88-98 | |
| IL-2 | EDTA Plasma (n=4) | 86 | 69-100 |
| Heparin Plasma (n=4) | 90 | 70-103 | |
| Serum (n=4) | 92 | 69-102 | |
| IFN-gamma | EDTA Plasma (n=4) | 89 | 88-90 |
| Heparin Plasma (n=4) | 90 | 83-99 | |
| Cerebrospinal Fluid (n=4) | 94 | 91-98 | |
| Serum (n=4) | 95 | 83-102 | |
| TNF-alpha | EDTA Plasma (n=4) | 97 | 89-105 |
| Heparin Plasma (n=4) | 89 | 77-96 | |
| Cerebrospinal Fluid (n=6) | 109 | 103-115 | |
| Serum (n=4) | 99 | 92-108 | |
| IL-4 | EDTA Plasma (n=4) | 88 | 83-92 |
| Heparin Plasma (n=4) | 87 | 84-89 | |
| Serum (n=4) | 96 | 94-99 | |
| IL-6 | EDTA Plasma (n=6) | 95 | 92-98 |
| Heparin Plasma (n=6) | 93 | 91-94 | |
| Serum (n=6) | 94 | 86-98 |
Specifications
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
Simple Plex Automated Immunoassay
Product Datasheets
Assay Procedure
IL-1b, IL-12 p70, IL-10, IL-2, IFNg 3rd gen, TNF-a 2nd gen, IL-4 2nd gen, IL-6 2nd genFAQs
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