Simple Plex Discovery Human GDNF Assay New

Catalog #: SPCKB-PS-012636 Datasheet / COA / SDS

Simple Plex Human GDNF assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

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SPCKB-PS-012636
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Simple Plex Discovery Human GDNF Ella Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human GDNF Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.103 pg/mL

Assay Range

0.7 - 2,748 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human GDNF.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Summary

Simple Plex Discovery assay is for the detection of human GDNF in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	24
Mean (pg/mL)	26.6	1123	21.3	1078
Standard Deviation	1.76	66.6	1.64	110
CV%	6.6	5.9	7.7	10.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	106	105-106
EDTA Plasma (n=2)	83	72-93
Heparin Plasma (n=2)	87	83-90
Serum (n=10)	81	68-94

Linearity

Samples containing and/or spiked with high concentrations of human GDNF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human GDNF Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDNF

GDNF (Glial Cell Line-derived Neurotrophic Factor) is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. It is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF binding to GFR alpha 1 induces the recruitment of Ret, NCAM-1/CD56, various integrins, Syndecan-3, or N-Cadherin. GDNF-based therapies show promise in several neurodegenerative disorders, particularly Parkinson’s disease.

Long Name:

Glial Cell line-derived Growth Factor

Entrez Gene IDs:

2668 (Human); 25453 (Rat)

Alternate Names:

Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3