Simple Plex Discovery Human Park7/DJ-1 Assay New

Catalog #: SPCKB-PS-012424 Datasheet / COA / SDS

Simple Plex Human Park7 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

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Simple Plex Discovery Human/Mouse/Rat Park7/DJ-1 Ella Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human Park7/DJ-1 Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), Mouse Serum (10 uL), Rat Serum (10 uL), EDTA Plasma (10 uL), Mouse EDTA Plasma (10 uL), Rat EDTA Plasma (10 uL), Heparin Plasma (10 uL), Mouse Heparin Plasma (10 uL), Rat Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

4.36 pg/mL

Assay Range

32.8 - 20,000 pg/mL (Cell Culture Supernates, Serum, Mouse Serum, Rat Serum, EDTA Plasma, Mouse EDTA Plasma, Rat EDTA Plasma, Heparin Plasma, Mouse Heparin Plasma, Rat Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant Park7.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human, mouse, and rat Parkinson Disease Gene 7 (Park7) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, Mouse Serum, Rat Serum, EDTA Plasma, Mouse EDTA Plasma, Rat EDTA Plasma, Heparin Plasma, Mouse Heparin Plasma, Rat Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	33	33
Mean (pg/mL)	141	6963	153	7341
Standard Deviation	8.65	411	11.9	486
CV%	6.1	5.9	7.8	6.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	98	91-105

Linearity

Samples containing and/or spiked with high concentrations of Park7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Mouse Park7/DJ-1 Ella Assay Linearity

Simple Plex Discovery Rat Park7/DJ-1 Ella Assay Linearity

Scientific Data

N/A

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Simple Plex Discovery Human/Mouse/Rat Park7/DJ-1 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Park7/DJ-1

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson's disease.

Long Name:

Parkinson Disease 7

Entrez Gene IDs:

11315 (Human); 57320 (Mouse); 117287 (Rat)

Alternate Names:

DJ1; DJ-1; DJ1FLJ34360; EC 3.4; FLJ27376; FLJ92274; Oncogene DJ1; Park7; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; protein DJ-1