Home / Host Cell Protein/HCP / Simple Plex HEK 293 HCP 3G Cartridge

Simple Plex HEK 293 HCP 3G Cartridge

Catalog #: SPCKB-OT-007066 Datasheet / COA / SDS
Simple Plex Host Cell Protein/HCP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD19, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-OT-007066
Simple Plex HEK 293 HCP 3G Assay Standard Curve
3 Images
Product Details
FAQs
Reviews
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex HEK 293 HCP 3G Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Bioprocess Samples (25 uL)
Sensitivity
0.2 ng/mL
Assay Range
1.6 - 400 ng/mL (Bioprocess Samples)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of Human Embryonic Kidney Host Cell Protein (HEK 293 HCP 3G) in bioprocess samples.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 8 8 81 79
Mean (ng/mL) 3.16 147 3.36 141
Standard Deviation 0.38 7.3 0.48 14.9
CV% 12 5 14.3 10.6

Recovery

Recovery at two different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Bioprocess Samples (n=12) 111 100-123

Linearity

Samples containing and/or spiked with high concentrations of HEK 293 HCP 3G were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Simple Plex HEK 293 HCP 3G Assay Linearity

Scientific Data

N/A Simple Plex HEK 293 HCP 3G Assay Standard Curve View Larger

Simple Plex HEK 293 HCP 3G Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex HEK 293 HCP 3G Assay. Assay range is 1.64-400 ng/mL.

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

FAQs

  1. What is the minimum required dilution (MRD) for bio-process samples?

    • All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery. 

View all Simple Plex Assays FAQs

Reviews for Simple Plex HEK 293 HCP 3G Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex HEK 293 HCP 3G Cartridge and earn rewards!

Have you used Simple Plex HEK 293 HCP 3G Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review