Home / Angiopoietin-like Protein 3/ANGPTL3 / Simple Plex Human Angiopoietin-like 3 Cartridge

Simple Plex Human Angiopoietin-like 3 Cartridge

Catalog #: SPCKB-PS-008169 Datasheet / COA / SDS
Simple Plex Human Angiopoietin-like Protein 3/ANGPTL3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKA-PS-008169
SPCKB-PS-008169
SPCKC-PS-008169
Control Products Available
897057: Simple Plex Control for Human Angiopoietin-like 3
Human Angiopoietin-like 3 Ella Assay
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Angiopoietin-like 3 Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
4.21 pg/mL
Assay Range
15.7 - 24,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human ANGPTL3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Angiopoietin-like 3 (ANGPTL3) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 105 101-109

Linearity

Samples containing and/or spiked with high concentrations of human ANGPTL3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Human Angiopoietin-like 3 Ella Assay Linearity

Scientific Data

N/A Human Angiopoietin-like 3 Ella Assay Standard Curve View Larger

Human Angiopoietin-like 3 Ella Assay Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Entrez Gene IDs:
27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names:
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2

FAQs

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