Simple Plex Human CCL16/HCC-4 Cartridge

Catalog #: SPCKB-PS-000336 Datasheet / COA / SDS
Simple Plex Human CCL16/HCC-4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000336
SPCKA-PS-000336
SPCKC-PS-000336
Control Products Available
Human HCC-4 Simple Plex Assay
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Simple Plex Human CCL16/HCC-4 Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity
2.2 pg/mL
Assay Range
7.9 - 12,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human HCC-4.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human HCC-4 in serum and plasma (EDTA/Heparin)

Recovery

Recovery was not necessary as HCC-4 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human HCC-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human HCC-4 Simple Plex Assay Linearity

Scientific Data

Human HCC-4 Simple Plex Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL16/HCC-4

Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1.

Entrez Gene IDs:
6360 (Human)
Alternate Names:
C-C motif chemokine 16; CCL16; chemokine (C-C motif) ligand 16; Chemokine CC-4; Chemokine LEC; CKb12; HCC4; HCC-4; HCC-4Liver-expressed chemokine; LCC-1Lymphocyte and monocyte chemoattractant; LEC; liver CC chemokine-1; LMC; LMCSmall-inducible cytokine A16; monotactin-1; Mtn-1; NCC-4; NCC4IL-10-inducible chemokine; NCC-4ILINCK; new CC chemokine 4; SCYA16MGC117051; SCYL4; small inducible cytokine subfamily A (Cys-Cys), member 16

FAQs

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