Simple Plex Human CCL19/MIP-3 beta Cartridge

Catalog #: SPCKB-PS-002348 Datasheet / COA / SDS

Simple Plex Human CCL19/MIP-3 beta assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-002348
SPCKA-PS-002348
SPCKC-PS-002348

Control Products Available

899465: Simple Plex Control for Human CCL19/MIP-3 beta

Simple Plex Human MIP-3 beta Assay Standard Curve

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Product Details

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CCL19/MIP-3 beta Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)

Sensitivity

0.35 pg/mL

Assay Range

1.6 - 6,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MIP-3 beta.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Macrophage Inflammatory Protein 3 beta (MIP-3 beta ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one MIP-3 beta assay

Inter-Assay Precision (Precision between assays) Replicates of each MIP-3 beta control were tested in multiple assays performed by at least three technicians using two lots of reagents for human MIP-3 beta

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	37	36
Mean (pg/mL)	36.4	2055	34.8	1881
Standard Deviation	1.57	115	3.51	209
CV%	4.3	5.6	10.1	11.1

Recovery

Recovery for the human MIP-3 beta assays was evaluated at three different spiked concentrations of MIP-3 beta protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=3)	100	95-104
Heparin Plasma (n=3)	97	94-100
Serum (n=3)	101	100-103

Linearity

Samples containing and/or spiked with high concentrations of human MIP-3 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human MIP-3 beta Simple Plex Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human MIP-3 beta Assay. Assay range is 1.57-6000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL19/MIP-3 beta

Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).

Entrez Gene IDs:

6363 (Human); 24047 (Mouse); 362506 (Rat)

Alternate Names:

beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19