Simple Plex Human CD40 Ligand/TNFSF5 Cartridge New

Catalog #: SPCKB-PS-011041 Datasheet / COA / SDS

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SPCKB-PS-011041
SPCKA-PS-011041
SPCKC-PS-011041

Control Products Available

897160: Simple Plex Control for Human CD40 Ligand/TNFSF5

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All CD40 Ligand/TNFSF5 Simple Plex Assays

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CD40 Ligand/TNFSF5 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Platelet-poor EDTA Plasma (25 uL), Heparin Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL)

Sensitivity

1.47 pg/mL

Assay Range

3.5 - 5,262 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma)

Specificity

Natural and recombinant human CD40 Ligand.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human CD40 Ligand in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and platelet-poor plasma (EDTA/Heparin).

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	101	95-107
EDTA Plasma (n=4)	87	81-88
Heparin Plasma (n=4)	90	85-95
Platelet-poor EDTA Plasma (n=4)	96	93-101
Platelet-poor Heparin Plasma (n=4)	90	87-96

Linearity

Samples containing and/or spiked with high concentrations of human CD40 Ligand were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Human CD40 Ligand Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD40 Ligand/TNFSF5

CD40 Ligand, also known as TNFSF5, CD154, TRAP, and gp39, is a trimeric protein that is expressed in transmembrane and soluble forms. It is expressed by many immune cell types as well as by endothelial and smooth muscle cells of the vasculature. It binds to CD40 on antigen presenting cells, platelets, and endothelial and epithelial cells, leading to enhanced B cell activation and T cell dependent humoral immune responses. CD40 Ligand dysregulation contributes to immune pathology in AIDS, atherosclerosis, atherothrombosis, and restenosis.

Entrez Gene IDs:

959 (Human); 21947 (Mouse); 84349 (Rat)

Alternate Names:

CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5