Simple Plex Human CXCL11/I-TAC Cartridge

Catalog #: SPCKB-PS-000369 Datasheet / COA / SDS

Simple Plex Human CXCL11/I-TAC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD16, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000369
SPCKA-PS-000369
SPCKC-PS-000369

Control Products Available

898412: Simple Plex Control for Human CXCL11/I-TAC

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CXCL11/I-TAC Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.181 pg/mL

Assay Range

0.5 - 2,080 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human I-TAC.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human I-TAC in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	8	5
Mean (pg/mL)	22	1212	21.9	1164
Standard Deviation	0.809	83.9	1.31	173
CV%	3.7	6.9	6	14.9

Recovery

Recovery was not necessary as I-TAC shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human I-TAC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human I-TAC Simple Plex Assay Standard Curve

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Human I-TAC Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL11/I-TAC

Interferon-inducible T cell a chemoattractant (I-TAC), also known as SCYB9B, H174 and beta-R1, is a non-ELR motif-containing CXC chemokine. I-TAC shares 36% and 37% amino acid sequence homology with IP-10 and MIG, respectively. I-TAC is expressed at low levels in normal tissues, including thymus, spleen and pancreas.

Entrez Gene IDs:

6373 (Human); 56066 (Mouse)

Alternate Names:

beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11