Simple Plex Human D-dimer Cartridge

Catalog #: SPCKB-PS-001976 Datasheet / COA / SDS

Simple Plex Human D-dimer assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001976
SPCKA-PS-001976
SPCKC-PS-001976

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898583: Simple Plex Control for Human D-dimer

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex Human D-dimer Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL), Citrate Plasma (5 uL)

Sensitivity

36.4 pg/mL

Assay Range

142.0 - 540,890 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human D-Dimer.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Summary

Simple Plex assay for the detection of human D-Dimer in cell culture supernatant (CCS), serum, and plasma (Citrate/EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	34	34
Mean (Not Assigned)	3288	174738	3282	173674
Standard Deviation	226	8198	267	11649
CV%	6.9	4.7	8.1	6.7

Recovery

Recovery was not necessary as D-Dimer shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human D-Dimer were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay