Simple Plex Human D-dimer Cartridge

Catalog #: SPCKB-PS-001976 Datasheet / COA / SDS

Simple Plex Human D-dimer assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001976
SPCKA-PS-001976
SPCKC-PS-001976

Control Products Available

898583: Simple Plex Control for Human D-dimer

Simple Plex Human D-Dimer Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex Human D-dimer Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)

Sensitivity

36.4 pg/mL

Assay Range

142.0 - 540,890 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human D-Dimer.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human D-Dimer in cell culture supernatant (CCS), serum, and plasma (Citrate/EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one D-Dimer assay

Inter-Assay Precision (Precision between assays) Replicates of each D-Dimer control were tested in multiple assays performed by at least three technicians using two lots of reagents for human D-Dimer

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	34	34
Mean (Not Assigned)	3288	174738	3282	173674
Standard Deviation	226	8198	267	11649
CV%	6.9	4.7	8.1	6.7

Recovery

Recovery was not necessary as human D-Dimer shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human D-Dimer were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human D-Dimer Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human D-Dimer Assay. Assay range is 142-540,890 pg/mL.