Simple Plex Human Endothelin-1 Cartridge

Catalog #: SPCKB-PS-000265 Datasheet / COA / SDS

Simple Plex Human Endothelin-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000265
SPCKA-PS-000265
SPCKC-PS-000265

Control Products Available

898177: Simple Plex Control for Human Endothelin-1

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Endothelin-1 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.11 pg/mL

Assay Range

0.3 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Endothelin-1.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Endothelin-1 in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin)

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	95	90-102
Heparin Plasma (n=4)	91	81-98
Serum (n=4)	85	82-88

Linearity

Samples containing and/or spiked with high concentrations of human Endothelin-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human Endothelin-1 Simple Plex Assay Standard Curve

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Human Endothelin-1 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endothelin-1

Endothelin-1 (ET-1) is produced by many tissues including lung, kidney, brain, endocrine glands, and placenta. It is also expressed by several cell types including, mast cells, endothelial cells, epithelial and smooth muscle cells. The most prominent source is vascular endothelium. ET-1 is best known as a potent vasoconstrictor. Elevated blood ET-1 levels are associated with a variety of diseases, possibly as a function of a stress response.

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Entrez Gene IDs:

1906 (Human); 13614 (Mouse); 24323 (Rat)

Alternate Names:

ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME