Simple Plex Human Ferritin Cartridge

Catalog #: SPCKB-PS-001044 Datasheet / COA / SDS

Simple Plex Human Ferritin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001044
SPCKA-PS-001044
SPCKC-PS-001044

Control Products Available

898923: Simple Plex Control for Human Ferritin

Ella Automated ELISA Platform and Simple Plex Human Ferritin Assay

3 Images

Product Details

Supplemental Products

Reviews

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex Human Ferritin Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

2.9 pg/mL

Assay Range

16.6 - 63,240 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant human Ferritin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Ferritin cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Ferritin assay

Inter-Assay Precision (Precision between assays) Replicates of each Ferritin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Ferritin

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	32	32
Mean (µL)	355	20037	362	20915
Standard Deviation	12.3	1650	28.8	2318
CV%	3.5	8.2	8	11.1

Recovery

Recovery was not necessary as human Ferritin shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Ferritin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

View Larger

Simple Plex Human Ferritin Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Ferritin Assay. Assay range is 16.6-636,240 pg/mL.