Simple Plex Human FGF-23 Cartridge

Catalog #: SPCKB-PS-002231 Datasheet / COA / SDS

Simple Plex Human FGF-23 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-002231
SPCKA-PS-002231
SPCKC-PS-002231

Control Products Available

899303: Simple Plex Control for Human FGF-23

Ella Automated ELISA Platform and Simple Plex Human FGF-23 Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human FGF-23 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

1.06 pg/mL

Assay Range

3.8 - 36,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human FGF-23.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human FGF-23 in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FGF-23 assay

Inter-Assay Precision (Precision between assays) Replicates of each FGF-23 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FGF-23

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	29
Mean (pg/mL)	185	10098	187	10286
Standard Deviation	5.43	660	22.1	840
CV%	2.9	6.5	11.8	8.2

Recovery

Recovery for the human FGF-23 assays was evaluated at three different spiked concentrations of FGF-23 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	94	90-97
Heparin Plasma (n=4)	99	95-102
Serum (n=4)	102	94-108

Linearity

Samples containing and/or spiked with high concentrations of human FGF-23 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human FGF-23 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human FGF-23 Assay. Assay range is 3.78-36,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-23

FGF-23 (fibroblast growth factor 23) is one of three FGF family members that appear to play a role in endocrine metabolism. It is described as being a phosphatonin, a molecule that reduces circulating plasma phosphate. FGF-23 does this, in part, by blocking Vitamin D activation. Active Vitamin D promotes intestinal phosphate absorption.

Long Name:

Fibroblast Growth Factor 23

Entrez Gene IDs:

8074 (Human); 64654 (Mouse)

Alternate Names:

ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor