Simple Plex Human FGF-23 Cartridge

Catalog #: SPCKB-PS-002231 Datasheet / COA / SDS

Simple Plex Human FGF-23 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-002231
SPCKA-PS-002231
SPCKC-PS-002231

Control Products Available

899303: Simple Plex Control for Human FGF-23

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human FGF-23 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

1.06 pg/mL

Assay Range

3.8 - 36,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human FGF-23.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human FGF-23 in serum and plasma (EDTA/Heparin)

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	94	90-97
Heparin Plasma (n=4)	99	95-102
Serum (n=4)	102	94-108

Linearity

Samples containing and/or spiked with high concentrations of human FGF-23 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human FGF-23 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-23

FGF-23 (fibroblast growth factor 23) is one of three FGF family members that appear to play a role in endocrine metabolism. It is described as being a phosphatonin, a molecule that reduces circulating plasma phosphate. FGF-23 does this, in part, by blocking Vitamin D activation. Active Vitamin D promotes intestinal phosphate absorption.

Long Name:

Fibroblast Growth Factor 23

Entrez Gene IDs:

8074 (Human); 64654 (Mouse)

Alternate Names:

ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor